anti-MAP2cd antibody [2C4]
anti-MAP2cd antibody [2C4] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
|Product Description||Mouse Monoclonal antibody [2C4] recognizes MAP2cd|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-Fr, WB|
|Immunogen||Full length recombinant Human MAP2D protein.|
|Full Name||microtubule-associated protein 2|
|Alternate Names||MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Calculated MW||200 kDa|
|Buffer||PBS and 50% Glycerol.|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||microtubule-associated protein 2|
|Background||This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]|
|Function||The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt]|
|Research Area||Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody|
|PTM||Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).|
Images (3) Click the Picture to Zoom In
ARG10720 anti-MAP2cd antibody [2C4] ICC/IF image
Immunocytochemistry: Rat mixed neuron and glia cultures stained with ARG10720 anti-MAP2cd antibody [2C4] (red) and co-stained with chicken antibody to Vimentin (green); DNA (blue). ARG10720 anti-MAP2cd antibody [2C4] antibody reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while vimentin was visualized in astrocytes and fibroblasts.
ARG10720 anti-MAP2cd antibody [2C4] IHC-Fr image
Immunohistochemistry: Frozen section of Adult Rat hippocampus stained with ARG10720 anti-MAP2cd antibody [2C4] (green) at 1:5000 dilution and Chicken pAb to FOX2 (red) at 1:2000 dilution. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)
Clone 2C4 labels all MAP2 protein isotypes expressed in neuronal perikarya and dendrites. The FOX2 antibody stains the nuclei of most neuronal cells.
ARG10720 anti-MAP2cd antibody [2C4] WB image
Western blot: Rat whole brain, HeLa, SH-SY5Y, HEK293 and NIH/3T3 cell lysates stained with ARG52468 anti-Vimentin antibody (red) at 1:5000 dilution.
The blot was simultaneously stained with ARG10720 anti-MAP2cd antibody [2C4] (green) at 1:5000 dilution, revealing multiple bands around 280 kDa that correspond to full length MAP2a/b isotypes while the ~ 70 kDa bands are MAP2c/d isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.