ARG10720

anti-MAP2cd antibody [2C4]

anti-MAP2cd antibody [2C4] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
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anti-MAP2cd antibody [2C4] Mouse Monoclonal antibody [2C4] recognizes MAP2cd ARG10720 Arigo biolaboratories
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anti-MAP2cd antibody [2C4]

Overview

Product Description Mouse Monoclonal antibody [2C4] recognizes MAP2cd
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 2C4
Isotype IgG1
Target Name MAP2cd
Antigen Species Human
Immunogen Full length recombinant Human MAP2D protein.
Conjugation Un-conjugated
Full Name microtubule-associated protein 2
Alternate Names MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:5000 - 1:10000
IHC-Fr1:5000 - 1:10000
WB1:5000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 200 kDa

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 17756 Mouse MAP2

GeneID: 25595 Rat MAP2

GeneID: 4133 Human MAP2

Gene Symbol MAP2
Gene Full Name microtubule-associated protein 2
Background This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt]
Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody; Neuron Marker antibody; Mature Neuron Marker antibody; Neurite Marker antibody
PTM Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).

Images (3) Click the Picture to Zoom In

  • ARG10720 anti-MAP2cd antibody [2C4] ICC/IF image

    Immunocytochemistry: Rat mixed neuron and glia cultures stained with ARG10720 anti-MAP2cd antibody [2C4] (red) and co-stained with chicken antibody to Vimentin (green); DNA (blue). ARG10720 anti-MAP2cd antibody [2C4] antibody reveals strong cytoplasmic staining of dendrites and perikarya of neuronal cells, while vimentin was visualized in astrocytes and fibroblasts.

  • ARG10720 anti-MAP2cd antibody [2C4] IHC-Fr image

    Immunohistochemistry: Frozen section of Adult Rat hippocampus stained with ARG10720 anti-MAP2cd antibody [2C4] (green) at 1:5000 dilution and Chicken pAb to FOX2 (red) at 1:2000 dilution. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)

    Clone 2C4 labels all MAP2 protein isotypes expressed in neuronal perikarya and dendrites. The FOX2 antibody stains the nuclei of most neuronal cells.

  • ARG10720 anti-MAP2cd antibody [2C4] WB image

    Western blot: Rat whole brain, HeLa, SH-SY5Y, HEK293 and NIH/3T3 cell lysates stained with ARG52468 anti-Vimentin antibody (red) at 1:5000 dilution.

    The blot was simultaneously stained with ARG10720 anti-MAP2cd antibody [2C4] (green) at 1:5000 dilution, revealing multiple bands around 280 kDa that correspond to full length MAP2a/b isotypes while the ~ 70 kDa bands are MAP2c/d isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.

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Review for anti-MAP2cd antibody [2C4]

Application:WB

Sample:Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:7500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC