ARG21507

anti-MMP1 antibody [SB12e] (HRP)

anti-MMP1 antibody [SB12e] (HRP) for ELISA,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ICC/IF,Western blot and Human,Rabbit

Overview

Product Description HRP-conjugated Mouse Monoclonal antibody [SB12e] recognizes MMP1
Tested Reactivity Hu, Rb
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Human/Rabbit MMP1.
Host Mouse
Clonality Monoclonal
Clone SB12e
Isotype IgG2b, kappa
Target Name MMP1
Antigen Species Human
Immunogen Recombinant full length MMP-1
Conjugation HRP
Full Name matrix metallopeptidase 1
Alternate Names MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:2000 - 1:4000
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 54 kDa

Properties

Form Liquid
Buffer 50% PBS (pH 7.4) and 50% Glycerol
Stabilizer 50% Glycerol
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100009110 Rabbit MMP1

GeneID: 4312 Human MMP1

Swiss-port # P03956 Human Interstitial collagenase

Swiss-port # P13943 Rabbit Interstitial collagenase

Gene Symbol MMP1
Gene Full Name matrix metallopeptidase 1
Background Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. [UniProt]
PTM Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Tyrosine phosphorylated in platelets by PKDCC/VLK.