ARG10732

anti-Neurofilament NF-L antibody antibody

anti-Neurofilament NF-L antibody antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Neurofilament NF-L antibody
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Neurofilament NF-L
Species Human
Immunogen Recombinant Human NF-L protein
Conjugation Un-conjugated
Full Name neurofilament, light polypeptide
Alternate Names Neurofilament triplet L protein; 68 kDa neurofilament protein; CMT1F; NF68; NFL; CMT2E; Neurofilament light polypeptide; NF-L; PPP1R110

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:5000
IHC-Fr1:5000
WB1:10000 - 1:15000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 62 kDa

Properties

Form Liquid
Purification Unpurified.
Buffer Serum.
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18039 Mouse NEFL

GeneID: 4747 Human NEFL

GeneID: 83613 Rat NEFL

Gene Symbol NEFL
Gene Full Name neurofilament, light polypeptide
Background Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. [UniProt]
Research Area Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Neuronal Cytoskeletal antibody; Neurofilament antibody; Intermediate Neurofilament antibody
PTM O-glycosylated.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Ubiquitinated in the presence of TRIM2 and UBE2D1.

Images (4) Click the Picture to Zoom In

  • ARG10732 anti-Neurofilament NF-L antibody antibody ICC/IF image

    Immunocytochemistry: Mixed neuron / glia cultures from newborn Rat brain stained with monoclonal 7C5 to peripherin (green) and co-stained with ARG10732 anti-Neurofilament NF-L antibody antibody (red). A class of large neurons, like the one in the middle of this image, contains peripherin, while the majority of neurons and their processes contain NF-L and not peripherin. Interestingly, the peripherin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both peripherin and NF-L, and so appears golden in this image. The blue channel reveals the localization of DNA.

  • ARG10732 anti-Neurofilament NF-L antibody antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Mouse cerebellum stained with ARG10732 anti-Neurofilament NF-L antibody (red) at 1:5000 dilution and costained with ARG52344 anti-Myelin Basic Protein antibody (green) at 1:5000 dilution. (Sample preparation: Following transcardial perfusion of Mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)

    The NF-L antibody labels dendrites and axons of neuronal cells, and Myelin Basic Protein antibody stains myelin sheathes around axons.

  • ARG10732 anti-Neurofilament NF-L antibody antibody WB image

    Western blot: Whole Rat brain homogenate stained with ARG10732 anti-Neurofilament NF-L antibody antibody  at 1:15000 dilution. A prominent band running with an apparent SDS-PAGE molecular weight of ~68 kDa corresponds to rodent NF-L.

  • ARG10732 anti-Neurofilament NF-L antibody antibody WB image

    Western blot: Rat brain, Rat spinal cord, Mouse brain and Mouse spinal cord lysates stained with ARG10732 anti-Neurofilament NF-L antibody (green) at 1:20000 dilution.