ARG10672

anti-OCT4 antibody

anti-OCT4 antibody for Flow cytometry,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes OCT4
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name OCT4
Antigen Species Human
Immunogen OCT3/4 recombinant protein fragment corresponding to a region within aa. 1 and aa. 360.
Conjugation Un-conjugated
Full Name POU class 5 homeobox 1
Alternate Names OTF-3; OCT4; OCT3; Octamer-binding protein 4; OTF3; Oct-3; Oct-4; OTF4; POU domain, class 5, transcription factor 1; Octamer-binding transcription factor 3; Octamer-binding protein 3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
ICC/IF1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS (pH 7.0), 0.01% Thimerosal, 20% Glycerol and 1% BSA.
Preservative 0.01% Thimerosal
Stabilizer 20% Glycerol and 1% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18999 Mouse POU5F1

GeneID: 5460 Human POU5F1

Swiss-port # P20263 Mouse POU domain, class 5, transcription factor 1

Swiss-port # Q01860 Human POU domain, class 5, transcription factor 1

Gene Symbol POU5F1
Gene Full Name POU class 5 homeobox 1
Background This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. [UniProt]
PTM Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

Images (1) Click the Picture to Zoom In

  • ARG10672 anti-OCT4 antibody ICC/IF image

    Immunocytochemistry: H1 human ES cells stained with ARG10672 anti-OCT4 antibody (green) at 1:100 dilution. Nuclei were counterstained with DAPI (blue).