anti-PINK1 antibody

anti-PINK1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody


Product Description

Mouse Monoclonal antibody recognizes PINK1

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 38CT20.8.5
Isotype IgG1
Target Name PINK1
Antigen Species Human
Immunogen Recombinant Human PINK1 protein.
Conjugation Un-conjugated
Alternate Names PARK6; BRPK; PTEN-induced putative kinase protein 1; Serine/threonine-protein kinase PINK1, mitochondrial; EC

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:100 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431


Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 65018 Human PINK1

GeneID: 68943 Mouse PINK1

Swiss-port # Q99MQ3 Mouse Serine/threonine-protein kinase PINK1, mitochondrial

Swiss-port # Q9BXM7 Human Serine/threonine-protein kinase PINK1, mitochondrial

Gene Symbol PINK1
Gene Full Name PTEN induced putative kinase 1
Background This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]
Function Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form. [UniProt]
Cellular Localization Mitochondrion outer membrane; Single-pass membrane protein. Cytoplasm, cytosol
Highlight Related products:
PINK1 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Astrocyte-to-neuron conversion for Parkinson's disease treatment
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 63 kDa
PTM Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria.
Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol.

Images (3) Click the Picture to Zoom In

  • ARG55419 anti-PINK1 antibody ICC/IF image

    Immunofluorescence: PC12 cells stained with ARG55419 anti-PINK1 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

  • ARG55419 anti-PINK1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart section stained with ARG55419 anti-PINK1 antibody at 1:25 dilution.

  • ARG55419 anti-PINK1 antibody WB image

    Western blot: 20 µg of A431 cell lysate stained with ARG55419 anti-PINK1 antibody at 1:1000 dilution.