ARG10088

anti-PSMA antibody [YPSMA-1]

anti-PSMA antibody [YPSMA-1] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human

Cancer antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [YPSMA-1] recognizes PSMA

Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Little or no cross-reactivity to benign prostate hyperplasia (BPH) or to normal prostatic tissue.
Host Mouse
Clonality Monoclonal
Clone YPSMA-1
Isotype IgG2b,k
Target Name PSMA
Antigen Species Human
Immunogen Crude membrane protein preparation from pooled prostate malignant carcinoma from China.
Conjugation Un-conjugated
Alternate Names FGCP; GCP2; PSM; Glutamate carboxypeptidase II; PSMA; NAALADase I; Membrane glutamate carboxypeptidase; Prostate-specific membrane antigen; FOLH; N-acetylated-alpha-linked acidic dipeptidase I; GCPII; Glutamate carboxypeptidase 2; NAALAdase; NAALAD1; Folate hydrolase 1; EC 3.4.17.21; Folylpoly-gamma-glutamate carboxypeptidase; mGCP; Cell growth-inhibiting gene 27 protein; Pteroylpoly-gamma-glutamate carboxypeptidase

Application Instructions

Application Suggestion
Application Dilution
ELISAAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note Immuno-histochemistry: This antibody was used on formalin-fixed paraffin embedded and frozen tissue sections.

Indirect ELISA: This antibody is reactive to PSMA expressing LNCap cells coated ELISA plate.

Western blot:The antibody, when used at 1μg/ml concentration, identified the 100KDa PSMA in LNcap cell extract.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.0)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 2346 Human FOLH1

Swiss-port # Q04609 Human Glutamate carboxypeptidase 2

Gene Symbol FOLH1
Gene Full Name folate hydrolase (prostate-specific membrane antigen) 1
Background Prostate specific membrane antigen (PSMA) is a type II trans-membrane glycoprotein found in the epithelium of prostate. It is also detectable in duodenal mucosa, renal tubules and some neuroendocrine cells. In other normal tissue, PSMA expression is not detectable. Because the expression of this membrane is elevated in malignant prostate tumour, PSMA has been used as a marker as well as a therapeutic target for prostate cancer. Elevated PSMA expression has also been identified in metastases of lymph nodes.
Function Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate. Isoform PSM-4 and isoform PSM-5 would appear to be physiologically irrelevant. Involved in prostate tumor progression.
Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. [UniProt]
Resrarch Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
PTM The first two amino acids at the N-terminus of isoform PSMA' appear to be cleaved by limited proteolysis.
The N-terminus is blocked.

Clone References

PSMA, EpCAM, VEGF and GRPR as imaging targets in locally recurrent prostate cancer after radiotherapy.

IHC-P / Human

Rybalov M et al.
Int J Mol Sci.,  (2014)

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Identification of a LNCaP-specific binding peptide using phage display.

WB / Human

Qin B et al.
Pharm Res.,  (2011)

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