ARG54096

anti-Pyruvate Dehydrogenase E2 antibody

anti-Pyruvate Dehydrogenase E2 antibody for Western blot,ICC/IF,Immunoprecipitation and Human,Mouse

Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody recognizes DLAT
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name Pyruvate Dehydrogenase E2
Antigen Species Human
Immunogen Purified recombinant human Pyruvate Dehydrogenase E2 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial; PDC-E2; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; EC 2.3.1.12; PBC; Pyruvate dehydrogenase complex component E2; DLTA; 70 kDa mitochondrial autoantigen of primary biliary cirrhosis; PDCE2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:300
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 69 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1737 Human DLAT

GeneID: 235339 Mouse DLAT

Swiss-port # P10515 Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydr

Swiss-port # Q8BMF4 Mouse Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydr

Gene Symbol DLAT
Gene Full Name dihydrolipoamide S-acetyltransferase
Background The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle. [UniProt]
Cellular Localization Mitochondrion matrix.
Research Area Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 69 kDa
PTM Delipoylated at Lys-132 and Lys-259 by SIRT4, delipoylation decreases the PHD complex activity.

Images (3) Click the Picture to Zoom In

  • ARG54096 anti-Pyruvate Dehydrogenase E2 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG54096 anti-Pyruvate Dehydrogenase E2 antibody at 1:300 dilution.

  • ARG54096 anti-Pyruvate Dehydrogenase E2 antibody WB image

    Western blot: Jurkat cell lysate stained with ARG54096 anti-Pyruvate Dehydrogenase E2 antibody at 1:1000 dilution.

  • ARG54096 anti-Pyruvate Dehydrogenase E2 antibody IP image

    Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54096 anti-Pyruvate Dehydrogenase E2 antibody.