ARG10656

anti-Rheb antibody [Rheb 3H6]

anti-Rheb antibody [Rheb 3H6] for Western blot,Immunoprecipitation and Human

Overview

Product Description Mouse Monoclonal antibody [Rheb 3H6] recognizes Rheb
Tested Reactivity Hu
Tested Application IP, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name Rheb
Antigen Species Human
Immunogen GST-tagged Human Rheb protein.
Conjugation Un-conjugated
Full Name Ras homolog enriched in brain
Alternate Names GTP-binding protein Rheb; RHEB2; Ras homolog enriched in brain

Application Instructions

Application Suggestion
Tested Application Dilution
IP5 µg/ml
WB1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6009 Human RHEB

Swiss-port # Q15382 Human GTP-binding protein Rheb

Gene Symbol RHEB
Gene Full Name Ras homolog enriched in brain
Background This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008]
Function Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity. [UniProt]
PTM Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation.