ARG10656
anti-Rheb antibody [Rheb 3H6]
anti-Rheb antibody [Rheb 3H6] for Western blot,Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody [Rheb 3H6] recognizes Rheb |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | Rheb |
| Antigen Species | Human |
| Immunogen | GST-tagged Human Rheb protein. |
| Conjugation | Un-conjugated |
| Alternate Names | GTP-binding protein Rheb; RHEB2; Ras homolog enriched in brain |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RHEB |
| Gene Full Name | Ras homolog enriched in brain |
| Background | This gene is a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22. [provided by RefSeq, Jul 2008] |
| Function | Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity. [UniProt] |
| Calculated MW | 20 kDa |
| PTM | Farnesylation is important for efficiently activating mTORC1-mediated signaling. Phosphorylation by MAPKAPK5 impairs GTP-binding and inactivation. |
