ARG54011

anti-SMC1A antibody (N-term)

anti-SMC1A antibody (N-term) for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Cell Biology and Cellular Response antibody

Overview

Product Description Mouse Monoclonal antibody recognizes SMC1A
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name SMC1A
Antigen Species Human
Immunogen Purified recombinant human SMC1A(N-terminus) protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names CDLS2; SMC-1A; Structural maintenance of chromosomes protein 1A; SMC1alpha; SMC1; DXS423E; Sb1.8; SMC-1-alpha; SMC1L1; SB1.8; SMCB; SMC protein 1A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 143 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8243 Human SMC1A

Swiss-port # Q14683 Human Structural maintenance of chromosomes protein 1A

Gene Symbol SMC1A
Gene Full Name structural maintenance of chromosomes 1A
Background Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interac tion with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
Function Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. [UniProt]
Cellular Localization Nucleus. Chromosome
Research Area Cell Biology and Cellular Response antibody
Calculated MW 143 kDa
PTM Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.

Images (3) Click the Picture to Zoom In

  • ARG54011 anti-SMC1A antibody (N-term) FACS image

    Flow Cytometry: HeLa cells stained with ARG54011 anti-SMC1A antibody (N-term) at 1:100 dilution (right) or isotype control antibody (left), followed by incubation with FITC labelled secondary antibody.

  • ARG54011 anti-SMC1A antibody (N-term) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon tissue stained with ARG54011 anti-SMC1A antibody (N-term) at 1:500 - 1:1000 dilution.

  • ARG54011 anti-SMC1A antibody (N-term) WB image

    Western blot: A431 cell lysate stained with ARG54011 anti-SMC1A antibody (N-term) at 1:1000 dilution.