ARG54986

anti-SNAIL antibody

anti-SNAIL antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody recognizes SNAIL
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 118CT12.3.2
Isotype IgM,κ
Target Name SNAIL
Antigen Species Human
Immunogen Human SNAIL recombinant protein
Conjugation Un-conjugated
Full Name snail family zinc finger 1
Alternate Names SNAH; SNAIL; SNA; dJ710H13.1; Protein sna; Protein snail homolog 1; Zinc finger protein SNAI1; SLUGH2; SNAIL1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:10 - 1:50
WB1:100 - 1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NCI-H460

Properties

Form Liquid
Buffer Crude Ascites and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6615 Human SNAI1

Swiss-port # O95863 Human Zinc finger protein SNAI1

Gene Symbol SNAI1
Gene Full Name snail family zinc finger 1
Background The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
PTM Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.

Images (2) Click the Picture to Zoom In

  • ARG54986 anti-SNAIL antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human testis stained with ARG54986 anti-SNAIL antibody.

  • ARG54986 anti-SNAIL antibody WB image

    Western blot: 35 µg of NCI-H460 cell lysate stained with ARG54986 anti-SNAIL antibody.