ARG20588

anti-STAT1 antibody

anti-STAT1 antibody for ICC/IF,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [M249] recognizes STAT1
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application ICC/IF, WB
Specificity This antibody detects 84 and 91 kDa stat1 variants on SDS-PAGE immunoblots of human Jurkat and A431 cells.
Host Mouse
Clonality Monoclonal
Clone M249
Isotype IgG1
Target Name STAT1
Antigen Species Human
Immunogen Recombinant protein of Human Stat1. This sequence has high homology to the conserved Tyrsite in Rat and Mouse stat1 a and b.
Conjugation Un-conjugated
Full Name signal transducer and activator of transcription 1, 91kDa
Alternate Names ISGF-3; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84; CANDF7; IMD31A; IMD31B; IMD31C; STAT91

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 87 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA
Preservative 0.05% Sodium azide.
Stabilizer 50% Glycerol and 1 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 6772 Human STAT1

Swiss-port # P42224 Human Signal transducer and activator of transcription 1-alpha/beta

Gene Symbol STAT1
Gene Full Name signal transducer and activator of transcription 1, 91kDa
Background The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Function Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. [UniProt]
PTM Phosphorylated on tyrosine and serine residues in response to a variety of cytokines/growth hormones including IFN-alpha, IFN-gamma, PDGF and EGF. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Upon EGF stimulation, phosphorylation on Tyr-701 (lacking in beta form) by JAK1, JAK2 or TYK2 promotes dimerization and subsequent translocation to the nucleus. Growth hormone (GH) activates STAT1 signaling only via JAK2. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PRKCD induces apoptosis in response to DNA-damaging agents. Phosphorylated on tyrosine residues when PTK2/FAK1 is activated; most likely this is catalyzed by a SRC family kinase. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates interferon-mediated signaling. Upon viral infection or IFN induction, phosphorylation on Ser-708 occurs much later than phosphorylation on Tyr-701 and is required for the binding of ISGF3 on the ISREs of a subset of IFN-stimulated genes IKBKE-dependent. Phosphorylation at Tyr-701 and Ser-708 are mutually exclusive, phosphorylation at Ser-708 requires previous dephosphorylation of Tyr-701.
Sumoylated with SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
ISGylated.

Images (2) Click the Picture to Zoom In

  • ARG20588 anti-STAT1 antibody WB image

    Western blot: A431 cells untreated (lanes 1) or treated with EGF (100 nM) for 60 min (lanes 2). The blots were stained with ARG20588 anti-STAT1 antibody (lanes 1, 2).

  • ARG20588 anti-STAT1 antibody ICC/IF image

    Immunocytochemistry: A431 cells stained with ARG20588 anti-STAT1 antibody (Right). Corresponding phase image (Left).