ARG22319

anti-Smac / Diablo antibody [2D12]

anti-Smac / Diablo antibody [2D12] for ELISA,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [2D12] recognizes Smac / Diablo
Tested Reactivity Hu
Tested Application ELISA, WB
Specificity Human Smac / DIABLO
Host Mouse
Clonality Monoclonal
Clone 2D12
Isotype IgG2b, kappa
Target Name Smac / Diablo
Antigen Species Human
Immunogen Recombinant Smac/DIABLO
Conjugation Un-conjugated
Alternate Names Smac; Second mitochondria-derived activator of caspase; Diablo homolog, mitochondrial; SMAC; Direct IAP-binding protein with low pI; DFNA64

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA< 1 ug/ml
WB< 2 ug/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56616 Human DIABLO

Swiss-port # Q9NR28 Human Diablo homolog, mitochondrial

Gene Symbol DIABLO
Gene Full Name diablo, IAP-binding mitochondrial protein
Background This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. [UniProt]
Calculated MW 27 kDa
PTM Ubiquitinated by BIRC7/livin.

Images (1) Click the Picture to Zoom In

  • ARG22319 anti-Smac / Diablo antibody [2D12] WB image

    Western blot: Jurkat total cell lysates were resolved by electrophoresis, trnasferred to PVDF membrane, and stained with ARG22319 anti-Smac / Diablo antibody [2D12]. Secondary antibody: ARG22404 Goat anti-Mouse IgG antibody (HRP), pre-adsorbed.