ARG54330

anti-Smac / Diablo antibody

anti-Smac / Diablo antibody for Western blot,Immunohistochemistry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Smac / Diablo
Tested Reactivity Hu
Tested Application IHC, WB
Specificity This antibody recognizes human, mouse, and rat Smac (25kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Smac / Diablo
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa 225-239 of human Smac (accession no. AAF87716).
Conjugation Un-conjugated
Alternate Names Smac; Second mitochondria-derived activator of caspase; Diablo homolog, mitochondrial; SMAC; Direct IAP-binding protein with low pI; DFNA64

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human heart and Human ovary

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 56616 Human DIABLO

Swiss-port # Q9NR28 Human Diablo homolog, mitochondrial

Gene Symbol DIABLO
Gene Full Name diablo, IAP-binding mitochondrial protein
Background Inhibitors of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases has been identified and designated Smac/DIABLO. Smac is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. The N-terminal amino acids of Smac are required for binding to IAPs and for activation of caspases. Smac is expressed in a variety of human and mouse tissues.
Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 27 kDa
PTM Ubiquitinated by BIRC7/livin.

Images (2) Click the Picture to Zoom In

  • ARG54330 anti-Smac / Diablo antibody WB image

    Western blot: A: Human heart tissue lysate in the absence of blocking peptide; B: Human heart tissue lysate in the presence of blocking peptid stained with ARG54330 anti-Smac / Diablo antibody at 1 μg/ml dilution.

  • ARG54330 anti-Smac / Diablo antibody IHC image

    Immunohistochemistry: Human heart tissue lysate stained with ARG54330 anti-Smac / Diablo antibody at 5 μg/ml dilution.