ARG54712

anti-Src antibody

anti-Src antibody for ICC/IF,Western blot and Human,Mouse

Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody

Overview

Product Description

Mouse Monoclonal antibody recognizes Src

Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 17AT28
Target Name Src
Antigen Species Human
Immunogen Human Src recombinant protein (NP_005408.1).
Conjugation Un-conjugated
Alternate Names Proto-oncogene c-Src; ASV; p60-Src; c-SRC; Proto-oncogene tyrosine-protein kinase Src; SRC1; pp60c-src; EC 2.7.10.2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:10 - 1:50
WB1:100 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HT29

Properties

Purification Protein G purified
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20779 Mouse SRC

GeneID: 6714 Human SRC

Swiss-port # P05480 Mouse Neuronal proto-oncogene tyrosine-protein kinase Src

Swiss-port # P12931 Human Proto-oncogene tyrosine-protein kinase Src

Gene Symbol SRC
Gene Full Name SRC proto-oncogene, non-receptor tyrosine kinase
Background This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. Due to functional redundancy between members of the SRC kinase family, identification of the specific role of each SRC kinase is very difficult. SRC appears to be one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinase (PTK) families. Receptor clustering or dimerization leads to recruitment of SRC to the receptor complexes where it phosphorylates the tyrosine residues within the receptor cytoplasmic domains. Plays an important role in the regulation of cytoskeletal organization through phosphorylation of specific substrates such as AFAP1. Phosphorylation of AFAP1 allows the SRC SH2 domain to bind AFAP1 and to localize to actin filaments. Cytoskeletal reorganization is also controlled through the phosphorylation of cortactin (CTTN). [UniProt]
Cellular Localization Cell membrane. Mitochondrion inner membrane. Nucleus. Cytoplasm, cytoskeleton. Note=Localizes to focal adhesion sites following integrin engagement. Localization to focal adhesion sites requires myristoylation and the SH3 domain
Highlight Related Antibody Duos and Panels:
ARG30169 Src Family Protein Tyrosine Kinases Antibody Panel
ARG30170 Phospho Src Antibody Panel
Related products:
Src antibodies; Src ELISA Kits; Src Duos / Panels;
Research Area Cancer antibody; Gene Regulation antibody; Signaling Transduction antibody; Src Family Protein Tyrosine Kinases antibody
Calculated MW 60 kDa
PTM Myristoylated at Gly-2, and this is essential for targeting to membranes.
Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation. CDK5-mediated phosphorylation at Ser-75 targets SRC to ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. Phosphorylated by PTK2/FAK1; this enhances kinase activity. Phosphorylated by PTK2B/PYK2; this enhances kinase activity.
S-nitrosylation is important for activation of its kinase activity.
Ubiquitinated in response to CDK5-mediated phosphorylation. Ubiquitination mediated by CBLC requires SRC autophosphorylation at Tyr-419 and may lead to lysosomal degradation.

Images (2) Click the Picture to Zoom In

  • ARG54712 anti-SRC Antibody ICC/IF image

    Immunofluorescence: A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then stained with ARG54712 anti-SRC antibody (1:25, 1 h at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C).

  • ARG54712 anti-SRC Antibody WB image

    Western blot: 35 µg of HT29 cell lysate stained with ARG54712 anti-SRC antibody at 1:1000 dilution.