ARG22255

anti-TCP1 alpha antibody [23c]

anti-TCP1 alpha antibody [23c] for Western blot,ICC/IF,Immunoprecipitation and Dog,Mouse,Rat,Bovine,Hamster,Rabbit,Sheep

Overview

Product Description Rat Monoclonal antibody [23c] recognizes TCP1 alpha
Tested Reactivity Ms, Rat, Bov, Dog, Hm, Rb, Sheep
Tested Application ICC/IF, IP, WB
Specificity Detects ~60kDa. Also detects ~92kDa. The addition of an alanine (LDDA COOH) prevents binding in ELISA assays to immobilized synthetic peptide sequences.
Host Rat
Clonality Monoclonal
Clone 23c
Isotype IgG2c
Target Name TCP1 alpha
Antigen Species Mouse
Immunogen Purified recombinant Mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp 1b gene expressed in E. coli. Detects the COOH group.
Conjugation Un-conjugated
Alternate Names T-complex protein 1 subunit alpha; CCT1; D6S230E; TCP-1-alpha; CCTa; CCT-alpha

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~60 kDa and ~92 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol
Preservative 0.1% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21454 Mouse TCP1

GeneID: 24818 Rat TCP1

GeneID: 512043 Bovine TCP1

Gene Symbol Tcp1
Gene Full Name t-complex protein 1
Background The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010]
Function Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. [UniProt]
Cellular Localization Cytoplasm
Calculated MW 60 kDa

Images (3) Click the Picture to Zoom In

  • ARG22255 anti-TCP1 alpha antibody [23c] ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22255 anti-TCP1 alpha antibody [23c] (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22255 anti-TCP1 alpha antibody [23c] WB image

    Western blot: 1) MW ladder, 20 µg of 2) A431 lysate, and 3) HEK293 lysate stained with ARG22255 anti-TCP1 alpha antibody [23c] (60 min at RT). Block: 5% milk + TBST 1hr at RT.

  • ARG22255 anti-TCP1 alpha antibody [23c] ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22255 anti-TCP1 alpha antibody [23c] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.