ARG20442

anti-TNF alpha antibody

anti-TNF alpha antibody for Flow cytometry,Immunohistochemistry,Neutralizing,Western blot and Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes TNF alpha
Tested Reactivity Rat
Tested Application FACS, IHC, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TNF alpha
Species Rat
Immunogen Recombinant Rat TNF alpha protein (NP_036807.1)
Conjugation Un-conjugated
Full Name tumor necrosis factor
Alternate Names Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10-20 µg/ml
IHC10-20 µg/ml
Neut0.5 µg/ml
WB1-2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 26 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 1 mg/ml BSA, 1.5 mM Sodium azide and 50% Glycerol
Preservative 1.5 mM Sodium azide
Stabilizer 1 mg/ml BSA, 50% Glycerol
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 24835 Rat TNF

Swiss-port # P16599 Rat Tumor necrosis factor

Gene Symbol Tnf
Gene Full Name tumor necrosis factor
Background Tumor Necrosis Factor alpha (TNF-I±) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-I± is a 17 kDa protein containing 157 amino acid residues.
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Images (1) Click the Picture to Zoom In

  • ARG20442 anti-TNF alpha antibody WB image

    Western blot: loding of 1) 10 ng 2) 50 ng and 3) 250 ng recombinabt Rat TNF alpha stained with ARG20442 anti-TNF alpha antibody.