ARG20442
anti-TNF alpha antibody
anti-TNF alpha antibody for Flow cytometry,Immunohistochemistry,Neutralizing,Western blot and Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TNF alpha |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | FACS, IHC, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TNF alpha |
| Antigen Species | Rat |
| Immunogen | Recombinant Rat TNF alpha protein (NP_036807.1) |
| Conjugation | Un-conjugated |
| Full Name | tumor necrosis factor |
| Alternate Names | Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Calculated MW | 26 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 1 mg/ml BSA, 1.5 mM Sodium azide and 50% Glycerol |
| Preservative | 1.5 mM Sodium azide |
| Stabilizer | 1 mg/ml BSA, 50% Glycerol |
| Concentration | 0.5 mg/ml |
| Storage instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database links | |
|---|---|
| Gene Symbol | Tnf |
| Gene Full Name | tumor necrosis factor |
| Background | Tumor Necrosis Factor alpha (TNF-I±) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-I± is a 17 kDa protein containing 157 amino acid residues. |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| PTM | The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. |
Images (1) Click the Picture to Zoom In
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ARG20442 anti-TNF alpha antibody WB image
Western blot: loding of 1) 10 ng 2) 50 ng and 3) 250 ng recombinabt Rat TNF alpha stained with ARG20442 anti-TNF alpha antibody.
