anti-TNF alpha antibody
anti-TNF alpha antibody for Flow cytometry,Immunohistochemistry,Neutralizing,Western blot and Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
|Product Description||Rabbit Polyclonal antibody recognizes TNF alpha|
|Tested Application||FACS, IHC, Neut, WB|
|Target Name||TNF alpha|
|Immunogen||Recombinant Rat TNF alpha protein (NP_036807.1)|
|Full Name||tumor necrosis factor|
|Alternate Names||Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Calculated MW||26 kDa|
|Purification||Affinity purification with immunogen.|
|Buffer||PBS, 1 mg/ml BSA, 1.5 mM Sodium azide and 50% Glycerol|
|Preservative||1.5 mM Sodium azide|
|Stabilizer||1 mg/ml BSA, 50% Glycerol|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||tumor necrosis factor|
|Background||Tumor Necrosis Factor alpha (TNF-I±) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-I± is a 17 kDa protein containing 157 amino acid residues.|
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody|
|PTM||The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
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