ARG20442
anti-TNF alpha antibody
anti-TNF alpha antibody for Flow cytometry,Immunohistochemistry,Neutralizing,Western blot and Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes TNF alpha |
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Tested Reactivity | Rat |
Tested Application | FACS, IHC, Neut, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | TNF alpha |
Antigen Species | Rat |
Immunogen | Recombinant Rat TNF alpha protein (NP_036807.1) |
Conjugation | Un-conjugated |
Full Name | tumor necrosis factor |
Alternate Names | Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
Calculated MW | 26 kDa |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | PBS, 1 mg/ml BSA, 1.5 mM Sodium azide and 50% Glycerol |
Preservative | 1.5 mM Sodium azide |
Stabilizer | 1 mg/ml BSA, 50% Glycerol |
Concentration | 0.5 mg/ml |
Storage instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database links | |
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Gene Symbol | Tnf |
Gene Full Name | tumor necrosis factor |
Background | Tumor Necrosis Factor alpha (TNF-I±) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-I± is a 17 kDa protein containing 157 amino acid residues. |
Highlight | Related products: TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Anti-Rabbit IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines |
Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
PTM | The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. |
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