ARG20577

anti-TRAP1 antibody [Trap1-6]

anti-TRAP1 antibody [Trap1-6] for Western blot,ICC/IF and Human

Overview

Product Description Mouse Monoclonal antibody [Trap1-6] recognizes TRAP1
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone Trap1-6
Isotype IgG2AKappa
Target Name TRAP1
Antigen Species Human
Immunogen Recombinant protein of Human TRAP1.
Conjugation Un-conjugated
Full Name TNF receptor-associated protein 1
Alternate Names HSP90L; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1; TNFR-associated protein 1; Heat shock protein 75 kDa, mitochondrial; HSP 75; HSP75

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 80 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% azide and 50% Glycerol.
Preservative 0.09% azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 10131 Human TRAP1

Swiss-port # Q12931 Human Heat shock protein 75 kDa, mitochondrial

Gene Symbol TRAP1
Gene Full Name TNF receptor-associated protein 1
Background This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
Function Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG20577 anti-TRAP1 antibody [Trap1-6] ICC/IF image

    Immunocytochemistry: Localization of TRAP1 to mitochondria in PC-3-M cells. A) staining of mitochondria with MitoTracker dye. B) staining of nuclei with DAPI. C) Staining with ARG20577 anti-TRAP1 antibody [Trap1-6]. D) tricolor composite of images A-C.

  • ARG20577 anti-TRAP1 antibody [Trap1-6] WB image

    Western blot: heat shocked HeLa cells stained with ARG20577 anti-TRAP1 antibody [Trap1-6] at 1:1000 dilution.