ARG65724

anti-Vimentin antibody

anti-Vimentin antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
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anti-Vimentin antibody

Mouse Monoclonal antibody recognizes Vimentin

ARG65724 Arigo biolaboratories
4.0 out of 5 based on 4
anti-Vimentin antibody is rated 4.0 out of 5 by 4.

anti-Vimentin antibody

Overview

Product Description

Mouse Monoclonal antibody recognizes Vimentin

Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Mouse
Clonality Monoclonal
Isotype IgG
Target Name Vimentin
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide of Human Vimentin (NP_003371.2).
Conjugation Un-conjugated
Full Name vimentin
Alternate Names Vimentin; CTRCT30; HEL113

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50 - 1:100
ICC/IF1:200
IHC-P1:200
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A549, HeLa, NIH/3T3 and Mouse embryonic stem cell
Calculated MW 54 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer 1*TBS (pH 7.4), 0.05% Sodium azide, 0.5% BSA and 40% Glycerol
Preservative 0.05% Sodium azide
Stabilizer 0.5% BSA, 40% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22352 Mouse VIM

GeneID: 7431 Human VIM

GeneID: 81818 Rat VIM

Gene Symbol VIM
Gene Full Name vimentin
Background This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. [UniProt]
Research Area Cancer antibody; Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody
PTM Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Images (3) Click the Picture to Zoom In

  • ARG65724 anti-Vimentin antibody ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG65724 anti-Vimentin antibody (green) at 1:200 dilution.

    Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)

  • ARG65724 anti-Vimentin antibody WB image

    Western blot: 20 µg of 293T cell lysate stained with ARG65724 anti-Vimentin antibody at 1:2000 dilution.

  • ARG65724 anti-Vimentin antibody WB image

    Western blot: 30 µg of A549, H1299 and 3T3 cell lysates stained with ARG65724 anti-Vimentin antibody at 1:2000 dilution.

Customer's Feedback

nuts_pic      Average

Review for anti-Vimentin antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Excellent

Review for anti-Vimentin antibody

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:200

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nuts_pic      Good

Review for anti-Vimentin antibody

Application:WB

Sample:A549, H1299 and 3T3 cells

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:1 hour

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Good

Review for anti-Vimentin antibody

Application:WB

Sample:H1299 (meta lung cancer)

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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