ARG20304

anti-YFP antibody

anti-YFP antibody for ICC/IF,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Gene Regulation antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes YFP
Tested Reactivity Other
Tested Application ICC/IF, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name YFP
Immunogen Recombinant YFP expressed from E. coli
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IP10-20 µg/ml
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 40365351 Other

Background Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. YFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or YFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or YFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Highlight Related Antibody Duos and Panels:
ARG30062 Tag Internal Control Antibody Duo (YFP, GAPDH)
ARG30209 Fluorescent-Tags Antibody Panel
Related products:
YFP antibodies; YFP Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Controls and Markers antibody; Gene Regulation antibody; Tag Internal Control antibody; Fluorescent-Tags antibody

Images (1) Click the Picture to Zoom In

  • ARG20304 anti-YFP antibody WB image

    Western Blot: 1. Recombinant YFP (10 ng) 2. Recombinant YFP (50 ng) 3. Recombinant YFP (250 ng) 4. Recombinant YFP (1000 ng) stained with anti-YFP antibody (ARG20304).