ARG62351

anti-alpha smooth muscle Actin antibody [1A4] (FITC)

anti-alpha smooth muscle Actin antibody [1A4] (FITC) for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Rat

Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody

Overview

Product Description FITC-cojugated Mouse Monoclonal antibody [1A4] recognizes alpha smooth muscle Actin
Tested Reactivity Rat
Predict Reactivity Hu, Ms, Cat, Chk, Dog, Goat, Gpig, Sheep, Zfsh
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 1A4
Isotype IgG2a
Target Name alpha smooth muscle Actin
Antigen Species Human
Immunogen N-terminal synthetic decapeptide of α-smooth muscle actin.
Conjugation FITC
Full Name actin, alpha 2, smooth muscle, aorta
Alternate Names Cell growth-inhibiting gene 46 protein; MYMY5; ACTSA; Alpha-actin-2; Actin, aortic smooth muscle; AAT6

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human tonsil or appendix.
Calculated MW 42 kDa

Properties

Form Liquid
Purification Purified Antibody
Buffer 1X PBS and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 81633 Rat ACTA2

Swiss-port # P62738 Rat Actin, aortic smooth muscle

Gene Symbol ACTA2
Gene Full Name actin, alpha 2, smooth muscle, aorta
Background The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt]
Highlight Related products:
alpha smooth muscle Actin antibodies; alpha smooth muscle Actin Duos / Panels; Anti-Mouse IgG secondary antibodies;
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New antibody panels and duos for Tumor immune microenvironment
Research Area Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody; Cancer-associated fibroblast antibody; Smooth Muscle Marker antibody; CAF Marker antibody; Mural cell Marker antibody; Myofibroblast Marker antibody; Angiogenesis Study antibody; Microvascular Density Study antibody
PTM Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).

Clone References

Expression of insulin-like growth factor 2 receptor in corneal keratocytes during differentiation and in response to wound healing.

WB, ICC/IF / Human

Bohnsack RN et al.
Invest Ophthalmol Vis Sci.,  (2014)

publication_link

 

hr_line

Elevated CXCL1 expression in breast cancer stroma predicts poor prognosis and is inversely associated with expression of TGF-β signaling proteins.

ICC/IF / Human

Zou A et al.
BMC Cancer.,  (2014)

publication_link

 

hr_line

Interferon regulatory factor 7 protects against vascular smooth muscle cell proliferation and neointima formation.

ICC/IF / Rat

Huang L et al.
J Am Heart Assoc.,  (2014)

publication_link

 

hr_line

Induction of transforming growth factor beta receptors following focal ischemia in the rat brain.

IHC-FoFr / Rat

Pál G et al.
PLoS One.,  (2014)

publication_link

 

hr_line

c-Jun N terminal kinase modulates NOX-4 derived ROS production and myofibroblasts differentiation in human breast stromal cells.

WB, ICC/IF / Human

Tobar N et al.
BMC Cancer.,  (2014)

publication_link

 

hr_line

Three-dimensional culture environment increases the efficacy of platelet rich plasma releasate in prompting skin fibroblast differentiation and extracellular matrix formation.

ICC/IF / Human

Rothan HA et al.
Int J Med Sci.,  (2014)

publication_link

 

hr_line

Chronic hypoxia-inducible transcription factor-2 activation stably transforms juxtaglomerular renin cells into fibroblast-like cells in vivo.

Kurt B et al.
J Am Soc Nephrol.,  (2015)

publication_link

 

hr_line

Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis.

IHC-Fr / Human

Rizvi S et al.
J Biol Chem.,  (2014)

publication_link

 

hr_line

iRGD tumor-penetrating peptide-modified oncolytic adenovirus shows enhanced tumor transduction, intratumoral dissemination and antitumor efficacy.

Puig-Saus C et al.
Gene Ther.,  (2014)

publication_link

 

hr_line

Hepatocyte growth factor regulates the TGF-β1-induced proliferation, differentiation and secretory function of cardiac fibroblasts.

WB, ICC/IF / Rat

Yi X et al.
Int J Mol Med.,  (2014)

publication_link

 

hr_line

Stroma provides an intestinal stem cell niche in the absence of epithelial Wnts.

ICC/IF / Mouse

Kabiri Z et al.
Development.,  (2014)

publication_link

 

hr_line

Interferon regulatory factor 8 modulates phenotypic switching of smooth muscle cells by regulating the activity of myocardin.

Zhang SM et al.
Mol Cell Biol.,  (2014)

publication_link

 

hr_line

Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences.

IHC-P, FACS / 

Akamatsu T et al.
Fibrogenesis Tissue Repair.,  (2013)

publication_link

 

hr_line

Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation.

ICC/IF / Sheep

Barboni B et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Changes in the Secretory Profile of NSCLC-Associated Fibroblasts after Ablative Radiotherapy: Potential Impact on Angiogenesis and Tumor Growth.

FACS / Human

Hellevik T et al.
Transl Oncol.,  (2013)

publication_link

 

hr_line

Trp53 inactivation in the tumor microenvironment promotes tumor progression by expanding the immunosuppressive lymphoid-like stromal network.

IHC / Mouse

Guo G et al.
Cancer Res.,  (2013)

publication_link

 

hr_line

Survival and differentiation of mammary epithelial cells in mammary gland development require nuclear retention of Id2 due to RANK signaling.

WB / Dog

Kim NS et al.
Mol Cell Biol.,  (2011)

publication_link

 

hr_line

Spermatogonia-specific proteins expressed in prepubertal buffalo (Bubalus bubalis) testis and their utilization for isolation and in vitro cultivation of spermatogonia.

IHC / Bovine

Goel S et al.
Theriogenology.,  (2010)

publication_link

 

hr_line

Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration.

ICC/IF / Rhesus Monkey

Wakao S et al.
Exp Neurol.,  (2010)

publication_link

 

hr_line

Multipotential ability of primitive germ cells from neonatal pig testis cultured in vitro.

ICC/IF / Pig

Goel S et al.
Reprod Fertil Dev.,  (2009)

publication_link

 

hr_line

Osteopontin modulates angiotensin II-induced inflammation, oxidative stress, and fibrosis of the kidney.

WB / Pig

Wolak T et al.
Kidney Int.,  (2009)

publication_link

 

hr_line

Vulvar leiomyosarcoma in a cat.

IHC / Cat

Firat I et al.
J Feline Med Surg.,  (2007)

publication_link

 

hr_line