ARG10701

anti-c-Fos antibody [2H2]

anti-c-Fos antibody [2H2] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow,Horse,Pig

Overview

Product Description Mouse Monoclonal antibody [2H2] recognizes c-Fos
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 2H2
Isotype IgG1
Target Name c-Fos
Antigen Species Human
Immunogen Full length recombinant Human protein expressed in and purified from E. coli.
Conjugation Un-conjugated
Full Name FBJ murine osteosarcoma viral oncogene homolog
Alternate Names Proto-oncogene c-Fos; C-FOS; AP-1; Cellular oncogene fos; p55; G0/G1 switch regulatory protein 7

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100 - 1:500
IHC-Fr1:100 - 1:500
WB1:200 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100144486 Pig FOS

GeneID: 14281 Mouse FOS

GeneID: 2353 Human FOS

Gene Symbol FOS
Gene Full Name FBJ murine osteosarcoma viral oncogene homolog
Background The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
Function Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. [UniProt]
PTM Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity).
Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.

Images (7) Click the Picture to Zoom In

  • ARG10701 anti-c-Fos antibody [2H2] ICC/IF image

    Immunofluorescence: HeLa cells were serum-starved (left) or serum-starved and then treated with 20% fetal bovine serum (FBS) for 2 hours (right). Cells were then stained with ARG10701 anti-c-Fos antibody [2H2] (green) at 1:1000 dilution and costained with ARG52468 anti-Vimentin antibody (red). DAPI (blue) for nuclear staining.

    Serum starvation inhibits c-Fos expression, while treatment with FBS for 2 hours strongly stimulates c-Fos expression which localizes in the nucleus as shown.

  • ARG10701 anti-c-Fos antibody [2H2] IHC-Fr image

    Immunohistochemistry: Frozen section of Rat hippocampus stained with ARG10701 anti-c-Fos antibody [2H2] (red) and costained with ARG10712 anti-FOX3 / NeuN antibody (green). DAPI (blue) reveals nuclei of neurons and glia.

    The hippocampal neurons stain green for FOX3 / NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.

  • ARG10701 anti-c-Fos antibody [2H2] IHC-P image

    Immunohistochemistry: Mouse brain section (45 µM; fixed by transcardial perfusion with 4% paraformaldehyde) stained with ARG10701 anti-c-Fos antibody [2H2] using a standard HRP-DAB (horseradish peroxidase-3,3'-diaminobenzidine) staining technique. Cells expressing c-Fos show dark color in nucleus.

  • ARG10701 anti-c-Fos antibody [2H2] WB image

    Western blot: Top panel: 1) HeLa cells were serum-starved for 36 hours, and 2) Serum-starved HeLa cells were stimulated with 20% FBS (fetal bovine serum) for 2 hours. ARG10701 anti-c-Fos antibody [2H2] recognizes bands in the range of 50-65 kDa, which represent multiple forms of c-Fos. Serum starvation attenuates c-Fos expression, while 20% FBS strongly stimulates c-Fos expression. Bottom panel:  Blot was stripped and stained with a monoclonal antibody (Clone: 1D4) against GAPDH, used as loading control.

  • ARG10701 anti-c-Fos antibody [2H2] WB image

    Western blot: 1) HeLa cells in serum free media, 2) HeLa cells stimulated with 20% fetal bovine serum for 2 hours after 36 hours in serum free media, 3) Rat cortical neurons and 4) Rat cortical neurons treated with membrane depolarization buffer for 5 hours. Lysates were stained with ARG10701 anti-c-Fos antibody [2H2] (green) at 1:1000 dilution and Rabbit pAb to GAPDH (red) at 1:20000 dilution.

  • ARG10701 anti-c-Fos antibody [2H2] ICC/IF image

    Immunofluorescence: Rat brain neural cells (left) and the same cells treated with membrane deplorization buffer for 5 hours (right). This is a salt solution containing 170 mM Potassium which depolarizes and stimulates gene expression in neuronal cells but has no effect on glia. Cells were stained with ARG10701 anti-c-Fos antibody [2H2] (green) and ARG52312 anti-GFAP antibody (red). DAPI (blue) for nuclear staining.

  • ARG10701 anti-c-Fos antibody [2H2] IHC-Fr image

    Immunohistochemistry: Frozen sections of Mouse cortical stained with ARG10701 anti-c-Fos antibody [2H2] (red) and co-stained with our rabbit polyclonal anti Fox3 / NeuN antibody (green) using immuno-fluorescent confocal-microscopy. Neurons positive for c-Fos and Fox3 / NeuN appear to be yellow. Inset shows an enlarged image. Nuclei are stained with DAPI (blue).