ARG53368

anti-eNOS antibody

anti-eNOS antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes eNOS
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name eNOS
Antigen Species Human
Immunogen Synthetic peptide derived from C-terminus of human e-NOS.
Conjugation Un-conjugated
Alternate Names Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-Dependent
ICC/IFAssay-Dependent
IHC-FrAssay-Dependent
IHC-P1:70
IPAssay-Dependent
WB1:70
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Placenta

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18127 Mouse NOS3

GeneID: 24600 Rat NOS3

GeneID: 4846 Human NOS3

Gene Symbol NOS3
Gene Full Name nitric oxide synthase 3 (endothelial cell)
Background Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt]
Cellular Localization Cytoplasm
Highlight Related Antibody Duos and Panels:
ARG30247 Phospho eNOS Antibody Duo (Total, pY1177)
Related products:
eNOS antibodies; eNOS ELISA Kits; eNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 133 kDa
PTM Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

Images (1) Click the Picture to Zoom In

  • ARG53368 anti-Nitric Oxide Synthase-Endothelial (eNOS) antibody IHC-P image

    Immunohistochemistry: Human Placenta stained with Nitric Oxide Synthase-Endothelial (eNOS) antibody (ARG53368)