anti-eNOS antibody for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
|Product Description||Rabbit Polyclonal antibody recognizes eNOS|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB|
|Immunogen||Synthetic peptide derived from C-terminus of human e-NOS.|
|Alternate Names||Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 184.108.40.206; ECNOS|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Immunogen affinity purified|
|Buffer||PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide|
|Preservative||< 0.1% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||nitric oxide synthase 3 (endothelial cell)|
|Background||Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]|
|Function||Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt]
|Highlight||Related Antibody Duos and Panels:
ARG30247 Phospho eNOS Antibody Duo (Total, pY1177)
eNOS antibodies; eNOS ELISA Kits; eNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies;
|Research Area||Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody|
|Calculated MW||133 kDa|
|PTM||Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.|
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