ARG10574

anti-iNOS antibody [K13-A]

anti-iNOS antibody [K13-A] for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Monoclonal antibody [K13-A] recognizes iNOS
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Monoclonal
Clone K13-A
Isotype IgG
Target Name iNOS
Antigen Species Human
Immunogen Synthetic peptide derived from Human iNOS.
Conjugation Un-conjugated
Full Name nitric oxide synthase 2, inducible
Alternate Names HEP-NOS; Inducible NO synthase; INOS; Nitric oxide synthase, inducible; iNOS; Hepatocyte NOS; NOS2A; Peptidyl-cysteine S-nitrosylase NOS2; Inducible NOS; NOS; NOS type II; EC 1.14.13.39

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 10 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18126 Mouse NOS2

GeneID: 24599 Rat NOS2

GeneID: 4843 Human NOS2

Gene Symbol NOS2
Gene Full Name nitric oxide synthase 2, inducible
Background Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG10574 anti-iNOS antibody [K13-A] WB image

    Western blot: 50 µg of Mouse brain stained with ARG10574 anti-iNOS antibody [K13-A] at 1:2000 dilution.