ARG10516

anti-p53 antibody [Pab 240]

anti-p53 antibody [Pab 240] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Chicken,Cow,Dog,Hamster,Human,Monkey,Mouse,Rat,Syrian Hamster

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody

Overview

Product Description

Mouse Monoclonal antibody [Pab 240] recognizes p53

Tested Reactivity Hu, Ms, Rat, Chk, Cow, Dog, Hm, Mk, SyrHm
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The epitope of clone [Pab240] is RHSVV and the sequence corresponding to amino acids 213-217 of the human p53 protein (P04637). This antibody can be used to detect p53 Arg213His mutation in methods using undenatured samples in undenatured western blot, IP analysis and IHC sections, the [PAb240] antibody recognizes both mutant and wild-type p53 in denatured methods (Ex: denatured WB). [Gannon, 1990; Said, 1994; Bartek, 1991; Walker, 1991; Charles, 1992)
Host Mouse
Clonality Monoclonal
Clone Pab 240
Isotype IgG1
Target Name p53
Antigen Species Human
Immunogen Purified p53-b-galactosidase fusion protein containing p53 sequence within 14 to 389 aa (derived from the pSV53C p53 cDNA clone)
Epitope RHSVV
Conjugation Un-conjugated
Full Name tumor protein p53
Alternate Names TRP53; LFS1; Cellular tumor antigen p53; Phosphoprotein p53; Tumor suppressor p53; BCC7; Antigen NY-CO-13; P53

Application Instructions

Application Suggestion
Tested Application
FACS1-2 µg for 10^6  cells.
ICC/IF 5 µg/ml
IHC-Fr1:250 - 1:500
IHC-P5 - 10 µg/ml
IP10 µg/ml
WB1 - 5 µg/ml
Application Note IHC-P: Antigen Retrieval: Heat mediated in Citrate buffer (pH 6.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MDA-MB-231 cell line
Calculated MW 53 kDa

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 101833915 Hamster TP53

GeneID: 24842 Rat TP53

GeneID: 396200 Chicken TP53

Gene Symbol TP53
Gene Full Name tumor protein p53
Background p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas.
Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody
PTM Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence. Deacetylation by SIRT2 impairs its ability to induce transcription activation in a AKT-dependent manner.

Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylation at Ser-9 by HIPK4 increases repression activity on BIRC5 promoter. Phosphorylated on Thr-18 by VRK1. Phosphorylated on Ser-20 by CHEK2 in response to DNA damage, which prevents ubiquitination by MDM2. Phosphorylated on Ser-20 by PLK3 in response to reactive oxygen species (ROS), promoting p53/TP53-mediated apoptosis. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-33 by CDK7 in a CAK complex in response to DNA damage. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP. Phosphorylated by NUAK1 at Ser-15 and Ser-392; was intially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1 (PubMed:21317932). It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated by DYRK2 at Ser-46 in response to genotoxic stress. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.

Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.

May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.

Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation (PubMed:10722742, PubMed:12810724, PubMed:15340061, PubMed:17170702, PubMed:19880522). Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:10722742, PubMed:12810724, PubMed:20173098). Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation (PubMed:19536131). Deubiquitinated by USP10, leading to its stabilization (PubMed:20096447). Ubiquitinated by TRIM24, RFFL, RNF34 and RNF125, which leads to proteasomal degradation (PubMed:19556538). Ubiquitination by TOPORS induces degradation (PubMed:19473992). Deubiquitination by USP7, leading to stabilization (PubMed:15053880). Isoform 4 is monoubiquitinated in an MDM2-independent manner (PubMed:15340061). Ubiquitinated by RFWD2, which leads to proteasomal degradation (PubMed:19837670). Ubiquitination and subsequent proteasomal degradation is negatively regulated by CCAR2 (PubMed:25732823).

Monomethylated at Lys-372 by SETD7, leading to stabilization and increased transcriptional activation. Monomethylated at Lys-370 by SMYD2, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity. Lys-372 monomethylation prevents interaction with SMYD2 and subsequent monomethylation at Lys-370. Dimethylated at Lys-373 by EHMT1 and EHMT2. Monomethylated at Lys-382 by KMT5A, promoting interaction with L3MBTL1 and leading to repress transcriptional activity. Dimethylation at Lys-370 and Lys-382 diminishes p53 ubiquitination, through stabilizing association with the methyl reader PHF20. Demethylation of dimethylated Lys-370 by KDM1A prevents interaction with TP53BP1 and represses TP53-mediated transcriptional activation.

Sumoylated with SUMO1. Sumoylated at Lys-386 by UBC9. [UniProt]

Clone References

Mesenchymal-endothelial transition contributes to cardiac neovascularization.

IM, IHC / Mouse

Ubil E et al.
Nature.,  (2014)

publication_link

 

hr_line

Aluminium induced endoplasmic reticulum stress mediated cell death in SH-SY5Y neuroblastoma cell line is independent of p53.

WB / Human

Mustafa Rizvi SH et al.
PLoS One.,  (2014)

publication_link

 

hr_line

TP53 supports basal-like differentiation of mammary epithelial cells by preventing translocation of deltaNp63 into nucleoli.

ICC/IF / Human

Munne PM et al.
Sci Rep.,  (2014)

publication_link

 

hr_line

Identification and characterization of small molecules that inhibit nonsense-mediated RNA decay and suppress nonsense p53 mutations.

WB / 

Martin L et al.
Cancer Res.,  (2014)

publication_link

 

hr_line

Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome.

IHC-P / Mouse

Takemura N et al.
Nat Commun.,  (2014)

publication_link

 

hr_line

Pharmacological and molecular effects of platinum(II) complexes involving 7-azaindole derivatives.

WB, IHC-P / Mouse

Starha P et al.
PLoS One.,  (2014)

publication_link

 

hr_line

Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells.

Toko H et al.
J Biol Chem.,  (2014)

publication_link

 

hr_line