ARG10406

anti-proBNP antibody [11D1]

anti-proBNP antibody [11D1] for Western blot and Human

Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [11D1] recognizes proBNP
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 11D1
Isotype IgG1
Target Name proBNP
Antigen Species Human
Immunogen synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 28-45
Conjugation Un-conjugated
Full Name natriuretic peptide B
Alternate Names 4-27; Natriuretic peptides B; 1-28; 1-29; 5-32; BNP; 1-32; 1-30; BNP-32; 5-29; 2-31; 4-30; 4-31; 4-32; 3-29; Gamma-brain natriuretic peptide; 3-30; 5-31; 3-32; 4-29

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 4879 Human NPPB

Swiss-port # P16860 Human Natriuretic peptides B

Gene Symbol NPPB
Gene Full Name natriuretic peptide B
Background This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]
Function Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Signaling Transduction antibody
PTM The brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

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  • ARG10406 anti-proBNP antibody [11D1] WB image

    Western Blot: human recombinant NT-proBNP expressed in E. coli stained with 1)anti-proBNP antibody [15F11] (ARG10408); 2) anti-proBNP antibody [11D1] (ARG10406) in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis.

  • ARG10406 anti-proBNP antibody [11D1] WB image

    Western Blot: human recombinant proBNP expressed in mammalian cells stained with 1) anti-proBNP antibody [15F11] (ARG10408); 2) anti-proBNP antibody [11D1] (ARG10406) in Western blotting by different antibodies after Tricine-SDS gel electrophoresis. Anti-proBNP antibody [11D1] (ARG10406) does not recognize glycosylated proBNP expressed in the mammalian cell line.