ARG10408

anti-proBNP antibody [15F11]

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200 μg

329.00

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Overview

Product Description	Mouse Monoclonal antibody [15F11] recognizes proBNP
Tested Reactivity	Hu
Tested Application	ELISA, WB
Host	Mouse
Clonality	Monoclonal
Clone	15F11
Isotype	IgG2b
Target Name	proBNP
Antigen Species	Human
Immunogen	synthetic N-terminal pro brain natriuretic peptide (NT-proBNP), corresponding to amino acid residues 13-27
Conjugation	Un-conjugated
Full Name	natriuretic peptide B
Alternate Names	4-27; Natriuretic peptides B; 1-28; 1-29; 5-32; BNP; 1-32; 1-30; BNP-32; 5-29; 2-31; 4-30; 4-31; 4-32; 3-29; Gamma-brain natriuretic peptide; 3-30; 5-31; 3-32; 4-29

Application Instructions

Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Purification	Protein A affinity purified.
Buffer	PBS (pH 7.4) and 0.1% Sodium azide
Preservative	0.1% Sodium azide
Concentration	1.0-2.0 mg/ml
Storage instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links	GeneID: 4879 Human NPPB Swiss-port # P16860 Human Natriuretic peptides B
Gene Symbol	NPPB
Gene Full Name	natriuretic peptide B
Background	This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]
Function	Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. [UniProt]
Research Area	Cell Biology and Cellular Response antibody; Signaling Transduction antibody
PTM	The brain natriuretic peptide 32 form is cleaved at Pro-104 by the prolyl endopeptidase FAP (seprase) activity (in vitro).

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ARG10408 anti-proBNP antibody [15F11] WB image

Western Blot: human recombinant NT-proBNP expressed in E. coli stained with 1)anti-proBNP antibody [15F11] (ARG10408); 2) anti-proBNP antibody [11D1] (ARG10406) in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis.
ARG10408 anti-proBNP antibody [15F11] WB image

Western Blot: Human recombinant proBNP expressed in mammalian cells stained with 1) anti-proBNP antibody [15F11] (ARG10408); 2) anti-proBNP antibody [11D1] (ARG10406) in Western blotting by different antibodies after Tricine-SDS gel electrophoresis.