anti-ACE2 antibody [AC18F]
anti-ACE2 antibody [AC18F] for ELISA,Flow cytometry,Western blot and Human
|Product Description||Mouse Monoclonal antibody [AC18F] recognizes ACE2|
|Tested Application||ELISA, FACS, WB|
|Immunogen||Recombinant Human ACE2.|
|Alternate Names||Angiotensin-converting enzyme homolog; ACEH; Angiotensin-converting enzyme 2; Metalloprotease MPROT15; ACE-related carboxypeptidase; EC 22.214.171.124|
|Application Note||WB: Use ECL as WB substrate. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||FACS: HepG2|
|Purification||Purification with Protein G.|
|Purification Note||0.2 µm filter sterilized.|
|Buffer||PBS (pH 7.4)|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||angiotensin I converting enzyme 2|
|Background||The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]|
|Function||Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator (PubMed:10969042, PubMed:10924499, PubMed:11815627). Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency (PubMed:11815627). By cleavage of angiotensin II, may be an important regulator of heart function (PubMed:10969042, PubMed:10924499). By cleavage of angiotensin II, may also have a protective role in acute lung injury (By similarity). Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SL6A19 in intestine, regulating trafficking, expression on the cell surface, and its catalytic activity (PubMed:18424768, PubMed:19185582).
(Microbial infection) Acts as a receptor for SARS coronavirus/SARS-CoV.
(Microbial infection) Acts as a receptor for Human coronavirus NL63/HCoV-NL63. [UniProt]
|Cellular Localization||Processed angiotensin-converting enzyme 2: Secreted. Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Detected in both cell membrane and cytoplasm in neurons. [UniProt]|
ACE2 antibodies; ACE2 ELISA Kits; ACE2 recombinant proteins; Anti-Mouse IgG secondary antibodies;
HMGB1, a biomarker and therapeutic target in COVID-19
|Calculated MW||92 kDa|
|PTM||N-glycosylation on Asn-90 may limit SARS infectivity.
Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. [UniProt]
Images (2) Click the Picture to Zoom In
ARG42475 anti-ACE2 antibody [AC18F] WB image
Western blot: Human ACE2 (Fc protein), Human ACE2 (Ecto domain) (FLAG-tagged) and Human GITR (Fc protein) (negative control). The blots were stained with ARG42475 anti-ACE2 antibody [AC18F] at 1:2000 dilution.
ARG42475 anti-ACE2 antibody [AC18F] FACS image
Flow Cytometry: HepG2 cells were stained with ARG42475 anti-ACE2 antibody [AC18F] (red line). Control (black line).