TOP 3 WARBURG EFFECT must-haves
A: Jurkat, B: A549, C: U2OS
PKM2 is a glycolytic enzyme specifically over-expressed in cancer cells that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP.
Application: WB, IHC-P, IF/ICC
LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. Mutation of LDHA has been found to be related to certain cancer types.
Application: FACS, IHC, WB
GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells. Dysfunctional mutations may lead to GLUT1 deficiency syndrome, whereas overexpression of GLUT1 is a prognostic indicator for cancer.
Reactivity: Human, Mouse, Rat
Application: FACS, ICC/IF, WB, IHC-Fr, IHC-P