ARG10555

anti-B Raf (V600E) antibody [K21-F] (FITC)

anti-B Raf (V600E) antibody [K21-F] (FITC) for Flow cytometry and Human

Overview

Product Description FITC-conjugated Rabbit Monoclonal antibody [K21-F] recognizes B Raf (V600E)
Tested Reactivity Hu
Tested Application FACS
Host Rabbit
Clonality Monoclonal
Clone K21-F
Isotype IgG
Target Name B Raf (V600E)
Antigen Species Human
Conjugation FITC
Full Name B-Raf proto-oncogene, serine/threonine kinase
Alternate Names BRAF1; v-Raf murine sarcoma viral oncogene homolog B1; Proto-oncogene B-Raf; p94; Serine/threonine-protein kinase B-raf; RAFB1; EC 2.7.11.1; B-RAF1; NS7

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer Aqueous buffer solution, 0.05% Sodium azide and 10 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 10 mg/ml BSA
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 673 Human BRAF

Swiss-port # P15056 Human Serine/threonine-protein kinase B-raf

Gene Symbol BRAF
Gene Full Name B-Raf proto-oncogene, serine/threonine kinase
Background This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008]
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway. [UniProt]
PTM Phosphorylation at Ser-365 by SGK1 inhibits its activity.
Methylation at Arg-671 decreases stability and kinase activity.
Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

Images (2) Click the Picture to Zoom In

  • ARG10555 anti-B Raf (V600E) antibody [K21-F] (FITC) FACS image

    Flow Cytometry: HT29 cells were fixed, permeabilized and stained with ARG10555 anti-B Raf (V600E) antibody [K21-F] (FITC) (green, 10 µl for HT29 per test) or with an isotype control (black).

  • ARG10555 anti-B Raf (V600E) antibody [K21-F] (FITC) FACS image

    Flow Cytometry: Chronic lymphocytic leukemia cells were fixed, permeabilized and stained with ARG10555 anti-B Raf (V600E) antibody [K21-F] (FITC) (blue, 4 µl for lymphocytes per test) or with an isotype control (black).