ARG52716

anti-CD20 antibody

anti-CD20 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes CD20
Tested Reactivity Hu
Predict Reactivity Dog
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD20
Antigen Species Human
Immunogen Synthetic peptide from C-terminus of human CD20 protein.
Conjugation Un-conjugated
Alternate Names Bp35; LEU-16; B-lymphocyte surface antigen B1; B-lymphocyte antigen CD20; CD20; S7; CD antigen CD20; Leukocyte surface antigen Leu-16; B1; CVID5; Membrane-spanning 4-domains subfamily A member 1; MS4A2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC-P1:300
WB1:25
Application Note IHC-P:
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control B Cell Lymphoma, Tonsil, Raji

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 931 Human MS4A1

Swiss-port # P11836 Human B-lymphocyte antigen CD20

Background CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul 2008]
Function CD20 is a B-lymphocyte-specific membrane protein. It plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602). [UniProt]
Cellular Localization Cytoplasm, Membrane
Highlight Related products:
CD20 antibodies; CD20 ELISA Kits; CD20 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Lymphoma
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Exploring Antiviral Immune Response
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; B cell Marker antibody; Immature B Cell Marker antibody; Inflammatory Cell Marker antibody; Tumor-infiltrating Lymphocyte Study antibody
Calculated MW 33 kDa
PTM Phosphorylated. Might be functionally regulated by protein kinase(s).

Images (2) Click the Picture to Zoom In

  • ARG52716 anti-CD20 antibody WB image

    Western Blot: Raji stained with CD20 antibody (ARG52716)

  • ARG52716 anti-CD20 antibody IHC-P image

    Immunohistochemistry: Human Tonsil stained with CD20 antibody (ARG52716)

Specific References

Shigella flexneri Targets Human Colonic Goblet Cells by O Antigen Binding to Sialyl-Tn and Tn Antigens via Glycan-Glycan Interactions.

ICC/IF / Human

Elizabeth Ngoc Hoa Tran et al.
ACS Infect Dis.,  (2020)

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