ARG57245

anti-CD321 / JAM1 antibody [RM275]

anti-CD321 / JAM1 antibody [RM275] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Monoclonal antibody [RM275] recognizes CD321 / JAM1
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity This antibody reacts to Human JAM1 (Junctional adhesion molecule 1).
Host Rabbit
Clonality Monoclonal
Clone RM275
Isotype IgG
Target Name CD321 / JAM1
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human CD321 / JAM1.
Conjugation Un-conjugated
Full Name F11 receptor
Alternate Names JAM-1; JAM1; Junctional adhesion molecule A; CD antigen CD321; JCAM; JAM-A; Junctional adhesion molecule 1; PAM-1; JAM; JAMA; KAT; Platelet F11 receptor; Platelet adhesion molecule 1; CD321

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:2000 - 1:10000
WB1:250 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide, 50% Glycerol and 1% BSA.
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol and 1% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 50848 Human F11R

Swiss-port # Q9Y624 Human Junctional adhesion molecule A

Gene Symbol F11R
Gene Full Name F11 receptor
Background Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008]
Function Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. [UniProt]
PTM N-glycosylated.

Images (2) Click the Picture to Zoom In

  • ARG57245 anti-CD321 / JAM1 antibody [RM275] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human kidney tissue sections stained with ARG57245 anti-CD321 / JAM1 antibody [RM275] at 1:10000 dilution.

  • ARG57245 anti-CD321 / JAM1 antibody [RM275] WB image

    Western blot: HEK293 cell lysates stained with ARG57245 anti-CD321 / JAM1 antibody [RM275] at 1:250 dilution.