ARG54059

anti-IkB alpha antibody (N-term)

anti-IkB alpha antibody (N-term) for Western blot and Human,Mouse,Rat

Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
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Overview

Product Description Mouse Monoclonal antibody recognizes NFKBIA
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Mouse
Clonality Monoclonal
Target Name IkB alpha
Antigen Species Human
Immunogen Purified recombinant human IkB-alpha(N-terminus) protein fragments expressed in E.coli.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 36 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol NFKBIA
Background Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription
Cellular Localization Cytoplasm. Nucleus
Highlight Related Antibody Duos and Panels:
ARG30037 Phospho IKB alpha Antibody Duo (Total, pS32/36)
ARG30205 NFkB Activation Antibody Panel
Related products:
IkB alpha antibodies; IkB alpha Duos / Panels;
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Research Area Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody

Images (1) Click the Picture to Zoom In

  • ARG54059 anti-IkB alpha antibody (N-term) WB image

    Western blot: 3T3 cell lysate stained with ARG54059 anti-IkB alpha antibody (N-term) at 1:500 dilution.

Specific References

Downregulation of lncRNA NEAT1 alleviates sepsis-induced acute kidney injury

WB / Human

Yuhua Zhou et al.
Central European Journal of Immunology,  (2022)

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Effects and mechanisms of PSS-loaded nanoparticles on coronary microcirculation dysfunction in streptozotocin-induced diabetic cardiomyopathy rats.

WB / Rat

Mao Yongjun et al.
Biomed Pharmacother.,  (2019)

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