ARG40756

anti-KDM4A / JHDM3A antibody

anti-KDM4A / JHDM3A antibody for Western blot and Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes KDM4A / JHDM3A
Tested Reactivity Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name KDM4A / JHDM3A
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 825-1064 of Human KDM4A (NP_055478.2).
Conjugation Un-conjugated
Full Name lysine (K)-specific demethylase 4A
Alternate Names JMJD2; Lysine-specific demethylase 4A; JMJD2A; Jumonji domain-containing protein 2A; JHDM3A; TDRD14A; EC 1.14.11.-; JmjC domain-containing histone demethylation protein 3A

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse brain
Calculated MW 120 kDa
Observed Size ~ 115 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 230674 Mouse KDM4A

Swiss-port # Q8BW72 Mouse Lysine-specific demethylase 4A

Gene Symbol KDM4A
Gene Full Name lysine (K)-specific demethylase 4A
Background This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009]
Function Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.

Isoform 2: Crucial for muscle differentiation, promotes transcriptional activation of the Myog gene by directing the removal of repressive chromatin marks at its promoter. Lacks the N-terminal demethylase domain. [UniProt]
Cellular Localization Nucleus. [UniProt]
Highlight Related products:
KDM4A antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Hypoxia-induced transcription, histone demethylases are involved
PTM Ubiquitinated by RNF8 and RNF168 following DNA damage, leading to its degradation. Degradation promotes accessibility of H4K20me2 mark for DNA repair protein TP53BP1, which is then recruited. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG40756 anti-KDM4A / JHDM3A antibody WB image

    Western blot: 25 µg of Mouse brain lysate stained with ARG40756 anti-KDM4A / JHDM3A antibody at 1:1000 dilution.