anti-Ku80 antibody

anti-Ku80 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

publication_link Publication1


Product Description Rabbit Polyclonal antibody recognizes Ku80
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Ku80
Antigen Species Human
Immunogen Recombinant protein of Human Ku80.
Conjugation Un-conjugated
Alternate Names double-strand-break rejoining; Thyroid-lupus autoantigen; Nuclear factor IV; KU80; DNA repair protein XRCC5; KARP1; Lupus Ku autoantigen protein p86; EC 3.6.4.-; CTCBF; CTC85; ATP-dependent DNA helicase 2 subunit 2; X-ray repair cross-complementing protein 5; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; KARP-1; X-ray repair complementing defective repair in Chinese hamster cells 5; 86 kDa subunit of Ku antigen; KUB2; NFIV; TLAA; Ku80; Ku86

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 7520 Human XRCC5

Swiss-port # P13010 Human X-ray repair cross-complementing protein 5

Gene Symbol XRCC5
Gene Full Name X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
Background The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
Function Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt]
Cellular Localization Chromosome, Nucleus, nucleolus. [UniProt]
Calculated MW 83 kDa
PTM Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.


Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites (PubMed:22266820). Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks (PubMed:26502055). [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG57867 anti-Ku80 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate cancer stained with ARG57867 anti-Ku80 antibody at 1:100 dilution.

  • ARG57867 anti-Ku80 antibody WB image

    Western blot: 25 µg of HepG2 cell lysate stained with ARG57867 anti-Ku80 antibody at 1:1000 dilution.

Specific References

miR-3059-3p Regulates Glioblastoma Multiforme Radiosensitivity Enhancement through the Homologous Recombination Pathway of DNA Repair

WB / Human

Yu-Wen Cheng et al.
J Oncol,  (2022)