ARG46147

anti-LMAN1 antibody

anti-LMAN1 antibody for Western blot and Human,Mouse

Overview

Product Description Mouse Monoclonal antibody recognizes LMAN1
Tested Reactivity Hu, Ms
Tested Application WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name LMAN1
Antigen Species Human
Immunogen Synthetic peptide near the carboxy terminus of human LMAN1.
Conjugation Un-conjugated
Alternate Names LMAN1; F5F8D; ERGIC-53; ER-Golgi intermediate compartment 53 kDa protein; FMFD1; Protein ERGIC-53; MR60; gp58; ERGIC53; Lectin mannose-binding 1; Intracellular mannose-specific lectin MR60; MCFD1; Gp58

Application Instructions

Application Suggestion
Tested Application Dilution
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Purification with Protein A
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3998 Human LMAN1

GeneID: 70361 Mouse LMAN1

Swiss-port # P49257 Human Protein ERGIC-53

Swiss-port # Q9D0F3 Mouse Protein ERGIC-53

Gene Symbol LMAN1
Gene Full Name lectin, mannose-binding, 1
Background The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency. [provided by RefSeq, Jul 2015]
Function Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins. [UniProt]
Cellular Localization Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 56 kDa
PTM The N-terminal may be partly blocked. [UniProt]