ARG20041

anti-PARP (cleaved) antibody

anti-PARP (cleaved) antibody for Immunohistochemistry,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PARP (cleaved)
Tested Reactivity Hu
Tested Application IHC, WB
Specificity The antibody recognizes only the large fragment of PARP (89 kDa) and does not react with the full length PARP.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PARP (cleaved)
Species Human
Immunogen Synthetic peptide correspond to the N-terminal residues of the catalytic domain of human PARP
Conjugation Un-conjugated
Full Name poly (ADP-ribose) polymerase 1
Alternate Names EC 2.4.2.30; Poly[ADP-ribose] synthase 1; PPOL; ADPRT; ARTD1; NAD; PARP-1; ADPRT 1; Poly [ADP-ribose] polymerase 1; PARP; ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT1; pADPRT-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Camptothecin treated Jurkat cell lysate
Calculated MW 113 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 50% Glycerol, 1% BSA and 0.02% Thimerosal
Preservative 0.02% Thimerosal
Stabilizer 50% Glycerol, 1% BSA
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 142 Human PARP1

Swiss-port # P09874 Human Poly [ADP-ribose] polymerase 1

Gene Symbol PARP1
Gene Full Name poly (ADP-ribose) polymerase 1
Background PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Function Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP)
ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB)
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PARP antibodies; PARP Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
PTM Phosphorylated by PRKDC and TXK.
Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites (PubMed:19661379). ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1 (PubMed:28190768).
S-nitrosylated, leading to inhibit transcription regulation activity.

Images (1) Click the Picture to Zoom In

  • ARG20041 anti-PARP (cleaved) antibody WB image

    Western blot: 30 µg of HeLa untreated or treated with Staurosporine and stained with ARG20041 anti-PARP (cleaved) antibody at 1:500 dilution.