ARG20547

anti-PSD95 antibody [6G6] (AP)

anti-PSD95 antibody [6G6] (AP) for Immunohistochemistry,ICC/IF and Human,Mouse,Rat,Bovine

Neuroscience antibody

Overview

Product Description

AP-conjugated Mouse Monoclonal antibody [6G6] recognizes PSD95

Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ICC/IF, IHC
Specificity Detects a ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
Host Mouse
Clonality Monoclonal
Clone 6G6
Isotype IgG2a
Target Name PSD95
Antigen Species Rat
Immunogen Recombinant Rat PSD-95 (NP_062567.1)
Conjugation AP
Full Name discs, large homolog 4 (Drosophila)
Alternate Names Postsynaptic density protein 95; SAP90; PSD-95; Synapse-associated protein 90; PSD95; SAP-90; Disks large homolog 4

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 80 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 13385 Mouse DLG4

GeneID: 1742 Human DLG4

GeneID: 29495 Rat DLG4

Gene Symbol Dlg4
Gene Full Name discs, large homolog 4 (Drosophila)
Background Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex. These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins. Thus different PDZ domains of PSD-95 might be specialized for distinct functions. PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism.
Function Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. [UniProt]
Cellular Localization Cell Membrane, Cell Junction, Synapse
Research Area Neuroscience antibody
PTM Palmitoylation of isoform 1 is required for targeting to postsynaptic density.