ARG10671

anti-SOX2 antibody

anti-SOX2 antibody for Flow cytometry,ICC/IF and Human,Mouse

Overview

Product Description Rat Monoclonal antibody recognizes SOX2
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF
Host Rat
Clonality Monoclonal
Isotype IgG2a, kappa
Target Name SOX2
Antigen Species Human
Immunogen Human SOX2 recombinant protein.
Conjugation Un-conjugated
Full Name SRY (sex determining region Y)-box 2
Alternate Names Transcription factor SOX-2; MCOPS3; ANOP3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
ICC/IF1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS (pH 7.2) and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 20674 Mouse SOX2

GeneID: 6657 Human SOX2

Swiss-port # P48431 Human Transcription factor SOX-2

Swiss-port # P48432 Mouse Transcription factor SOX-2

Gene Symbol SOX2
Gene Full Name SRY (sex determining region Y)-box 2
Background This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). [UniProt]
PTM Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

Images (1) Click the Picture to Zoom In

  • ARG10671 anti-SOX2 antibody ICC/IF image

    Immunocytochemistry: H1 human ES cells stained with ARG10671 anti-SOX2 antibody (green) at 1:100 dilution. Nuclei were counterstained with DAPI (blue).