ARG10580

anti-p16 antibody [R19-D]

anti-p16 antibody [R19-D] for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Monoclonal antibody [R19-D] recognizes p16
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone R19-D
Isotype IgG
Target Name p16
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human p16
Conjugation Un-conjugated
Full Name cyclin-dependent kinase inhibitor 2A
Alternate Names Alternative reading frame; CDK4I; P19ARF; P16INK4A; CDKN2; Tumor suppressor ARF; MTS-1; P14; P16; P19; Cyclin-dependent kinase inhibitor 2A; P16INK4; MLM; p14ARF; MTS1; INK4; TP16; P16-INK4A; CMM2; ARF; P14ARF; INK4A

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 20 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 20 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 1029 Human CDKN2A

Swiss-port # P42771 Human Cyclin-dependent kinase inhibitor 2A

Gene Symbol CDKN2A
Gene Full Name cyclin-dependent kinase inhibitor 2A
Background This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq, Sep 2012]
Function Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform smARF may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP. [UniProt]
Highlight Related products:
p16 antibodies; p16 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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PTM Ubiquitinated in normal cells by TRIP12 via the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination at the N-terminus, regardeless of the absence of lysine residues. Ubiquitination leads to its proteasomal degradation. In cancer cells, however, TRIP12 is located in a different cell compartment, preventing ubiquitination and degradation.

Images (4) Click the Picture to Zoom In

  • ARG10580 anti-p16 antibody [R19-D] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human cervical lymph node metastasis of oropharyngeal HPV-associated squamous cell carcinoma (4 µm sections) stained with ARG10580 anti-p16 antibody [R19-D].

  • ARG10580 anti-p16 antibody [R19-D] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human high grade squamous intraepithelial lesion of the uterine cervix (in contrast with normal endocervical glands) (4 µm sections) stained with ARG10580 anti-p16 antibody [R19-D].

  • ARG10580 anti-p16 antibody [R19-D] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human high grade squamous intraepithelial lesion of the uterine cervix (in contrast with non-dysplastic metaplastic squamous epithelium) (4 µm sections) stained with ARG10580 anti-p16 antibody [R19-D].

  • ARG10580 anti-p16 antibody [R19-D] IHC-P image

    Immunohistochemistry: Formalin fixed, paraffin embedded Human adenocarcinoma in situ of the uterine cervix (4 µm sections) stained with ARG10580 anti-p16 antibody [R19-D].