ARG30105

Apoptosis Marker Antibody Duo (Caspase3, PARP)

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Neuroscience antibody
publication_link Publication1

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG20001 anti-Caspase 3 antibody [C33] Mouse mAb Hu WB 25 μg
ARG20041 anti-PARP (cleaved) antibody Rabbit pAb Hu IHC, WB 25 μg

Overview

Product Description

The TUNEL assay has been commonly used as a detection method for identifying apoptotic cells. While useful, it can also yield highly variable results as some non-apoptotic nuclei showing high levels of RNA synthesis/splicing can also be labeled by TUNEL technique.
Cleavage of Caspase 3 and PARP (Poly (ADP-ribose) Polymerase is a pre-requisite for cells who undergo final stage of cell death. The use of anti-cleaved caspase3 (ARG20003) and anti-cleaved PARP (ARG20041) antibodies adds a more specific weapon to the arsenal of techniques for confirming apoptosis in tissue specimens.

Target Name Apoptosis Marker
Alternate Names Apoptosis Marker antibody; Caspase 3 antibody; PARP (cleaved) antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Antibody Duo for Apoptosis Marker (Caspase3, PARP)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Neuroscience antibody

Images (4) Click the Picture to Zoom In

  • ARG20001 anti-Caspase 3 antibody [C33] WB image

    Western blot: 30 µg of HeLa cells untreated or treated with Staurosporine (1μM, overnight). The blots were stained with ARG20001 anti-Caspase 3 antibody [C33] at 1:500 dilution.

  • ARG20041 anti-PARP (cleaved) antibody WB image

    Western blot: 30 µg of HeLa untreated or treated with Staurosporine and stained with ARG20041 anti-PARP (cleaved) antibody at 1:500 dilution.

  • ARG20001 anti-Caspase-3 antibody [C33] WB image

    Western blot: HeLa cells treated with camptothecin (2 µM) stained with ARG20001 anti-Caspase-3 antibody [C33].

    The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. However, the optimal conditions should be determined individually.

  • ARG20001 anti-Caspase 3 antibody [C33] WB image

    Western blot: 20 µg of HeLa cell lysates stained with ARG20001 anti-Caspase 3 antibody [C33] at 2 µg/ml dilution.

Specific References

Inhibition of histone acetylation by curcumin reduces alcohol-induced fetal cardiac apoptosis.

ARG20001: WB / Mouse

Yan X et al.
J Biomed Sci.,  (2017)

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