ARG82158

Fumarase Activity Assay Kit (Colorimetric)

Fumarase Activity Assay Kit (Colorimetric) for Functional study and All species,Human,Mouse,Rat

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Overview

Product Description ARG82158 Fumarase Activity Assay Kit (Colorimetric) can be used to measure Fumarase activity in serum, plasma, erythrocytes, tissue and cell culture supernatants.
Tested Reactivity Hu, Ms, Rat, All
Tested Application FuncSt
Target Name Fumarase
Conjugation Note Read at 565 nm
Sensitivity 0.4 U/L
Detection Range 0.4 - 70 U/L
Sample Type serum, plasma, erythrocytes, tissue and cell culture supernatants
Sample Volume 20 µl
Alternate Names MCUL1; MCL; LRCC; EC 4.2.1.2; Fumarate hydratase, mitochondrial; FMRD; Fumarase; HLRCC

Application Instructions

Application Note Please note that this kit does not include a microplate.
Assay Time 40 min

Properties

Form Liquid
Storage Instruction Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2271 Human FH

Swiss-port # P07954 Human Fumarate hydratase, mitochondrial

Gene Symbol FH
Gene Full Name fumarate hydratase
Background The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
Function Also acts as a tumor suppressor. [UniProt]
Cellular Localization Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. [UniProt]
Highlight Related products:
Fumarase antibodies; Fumarase ELISA Kits;
Related news:
TCA intermediate fumarate promotes mitobiogenesis

Images (1) Click the Picture to Zoom In

  • ARG82158 Fumarase Activity Assay Kit (Colorimetric) enzyme kinetics graph

    Kinetics of 0 and 70 U/L Fumarase reaction, using ARG82158 Fumarase Activity Assay Kit (Colorimetric).

Specific References

Resolvin D1 reduces mitochondrial damage to photoreceptors of primary retinal cells exposed to high glucose.

FuncSt / Mouse

Trotta Maria Consiglia et al.
J Cell Physiol.,  (2019)

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