ARG82767

Glutaminase Assay Kit (Colorimetric)

Glutaminase Assay Kit (Colorimetric) for Functional study and All species

Overview

Product Description ARG82767 Glutaminase Assay Kit (Colorimetric) is a detection kit for the quantification of Glutaminase activity in cells, bacteria and tissue samples.
Tested Reactivity All
Tested Application FuncSt
Specificity The assay is initiated with the enzymatic hydrolysis of Glutamine by Glutaminase. The enzyme catalysed reaction products can be measured at a colorimetric readout at 420 nm.
Target Name Glutaminase
Conjugation Note Read at 420 nm (colorimetric)
Detection Range 0.01 - 1 µmol/ml
Sample Type Cells, bacteria and tissue samples.
Sample Volume 20 µl
Alternate Names EC 3.5.1.2; AAD20; L-glutamine amidohydrolase; KGA; Glutaminase kidney isoform, mitochondrial; GAM; K-glutaminase; GLS; GAC; GLS1

Application Instructions

Assay Time ~ 1.5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol GLS
Gene Full Name glutaminase
Background This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Function Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30575854, PubMed:30239721, PubMed:30970188).

[Isoform 2]: Lacks catalytic activity. [UniProt]
Cellular Localization Isoform 1: Cytoplasm, cytosol. Isoform 3: Mitochondrion. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG82767 Glutaminase Assay Kit (Colorimetric) typical data demonstration image

    ARG82767 Glutaminase Assay Kit (Colorimetric) results of a typical standard run with optical density reading at 420 nm, using colorimetric reading.

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