ARG82029
Glycolate Oxidase Assay Kit
Glycolate Oxidase Assay Kit for Functional study and All
Overview
| Product Description | ARG82029 Glycolate Oxidase Assay Kit is a detection kit for the quantification of Glycolate Oxidase Activity in tissue extracts, cell lysate and cell culture supernatants. |
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| Tested Reactivity | All |
| Tested Application | FuncSt |
| Target Name | Glycolate Oxidase |
| Conjugation Note | Read at 500 nm. |
| Sensitivity | 0.05 mmol/L |
| Detection Range | 0.05 - 5 mmol/L |
| Sample Type | Tissue extracts, cell lysate and cell culture supernatants. |
| Sample Volume | 20 µl |
| Alternate Names | EC 1.1.3.15; Glycolate oxidase; HAOX1; GOX; GOX1; Hydroxyacid oxidase 1 |
Application Instructions
| Assay Time | 15 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | HAO1 |
|---|---|
| Gene Full Name | hydroxyacid oxidase (glycolate oxidase) 1 |
| Background | This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008] |
| Function | Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate. [UniProt] |
| Cellular Localization | Peroxisome. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82029 Glycolate Oxidase Assay Kit typical data demonstration image
ARG82029 Glycolate Oxidase Assay Kit results of a typical data with optical density reading at 500 nm.
| Title | Download Link |
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| ARG82029 Glycolate Oxidase Assay Kit User manual |
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