ARG10539

Goat anti-Mouse IgG / IgA / IgM (H+L) antibody (TRITC)

Goat anti-Mouse IgG / IgA / IgM (H+L) antibody (TRITC) for ELISA,ICC/IF,IHC-Frozen sections and Guinea pig,Mouse,Rat

Immune System antibody

Overview

Product Description

TRITC-conjugated Goat Polyclonal antibody recognizes Mouse IgG / IgA / IgM (H+L)

Tested Reactivity Ms, Rat, Gpig
Species Does Not React With Hu, Bov, Cat, Chk, Dog, Hrs, Mk, Pgn, Pig, Rb, Sheep
Tested Application ELISA, ICC/IF, IHC-Fr
Specificity TRITC-conjugated IgG fraction of polyclonal Goat antiserum to Mouse IgG, IgA and IgM (H+L).
Host Goat
Clonality Polyclonal
Target Name Mouse IgG
Target Ig IgG / IgA / IgM (H+L)
Conjugation TRITC

Application Instructions

Cross Reactivity Note Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion anti several species sera with the following results: Bovine (-), Chicken (-), Duck (-), Hamster (±), Human (-), pigeon (-), Rat (+), Swine (-), Cat (-), Dog (-), Guinea Pig (+), Horse (-), Monkey (-), Rabbit (-), Sheep (-), Turkey (-). A negative cross-reaction in double radial immunodiffusion does not exclude some reaction in more sensitive techniques.
Application Suggestion
Tested Application Dilution
ELISA1:500 - 1:5,000
ICC/IF1:20 - 500
IHC-Fr1:20 - 500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Properties

Form Liquid
Buffer PBS (pH 7.2)
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Research Area Immune System antibody