ARG23804

Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed

Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed for ELISA,FLISA,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,ELISPOT,Purification and Mouse

Overview

Product Description AP-conjugated Goat Polyclonal antibody recognizes Mouse IgG2a
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB
Specificity Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Mouse IgG2a
Antigen Species Mouse
Conjugation AP

Application Instructions

Pre Adsorbed Mouse IgG1, IgG2b, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISA1:2000 - 1:4000
ELISPOTAssay-dependent
FACSAssay-dependent
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
PuriAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Tris (pH 8.0), 0.1% Sodium azide, 1 mM MgCl2 and 50% Glycerol.
Preservative 0.1% Sodium azide
Stabilizer 50% Glycerol
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (7) Click the Picture to Zoom In

  • ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 was stained with ARG22307 anti-Cytokeratin 8 antibody [SB37b] and ARG21506 anti-MMP1 antibody [SB12e] followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed and ARG21894 Goat anti-Mouse IgG2b antibody (HRP), pre-adsorbed, Red AP, DAB, and mounted with Fluoromount-G.

  • ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed IHC-P image

    Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue was stained with Mouse anti-Human CD44 followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed, Red AP, hematoxylin, and mounted with Fluoromount-G.

  • ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed ELISA image

    ELISA: ELISA plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed.

  • ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 was stained with ARG22307 anti-Cytokeratin 8 antibody [SB37b] followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed, Red AP, and mounted with Fluoromount-G.

  • ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse anti-Human EGFR (right) followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed, Red AP, and mounted with Fluoromount-G.

  • ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse anti-Human CD44 followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed, Red AP, and mounted with Fluoromount-G.

  • ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse anti-Human CD44 and Mouse anti-Acetyl-Histone H3 (Lys4) followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP), pre-adsorbed and ARG21894 Goat anti-Mouse IgG2b antibody (HRP), pre-adsorbed, Red AP, DAB, and mounted with Fluoromount-G.