ARG21533

Goat anti-Mouse IgM antibody (Biotin), pre-adsorbed

Goat anti-Mouse IgM antibody (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgM
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with the heavy chain of Mouse IgM. The antibody is pre-adsorbed with Mouse IgG and IgA, so the antibody may not react with Mouse IgG and IgA, but may react with immunoglobulins from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Mouse IgM
Antigen Species Mouse
Conjugation Biotin

Application Instructions

Pre Adsorbed Mouse IgG and IgA.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and Sodium azide
Preservative Sodium azide
Concentration 0.5 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21533 Goat anti-Mouse IgM antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted ARG21533 Goat anti-Mouse IgM antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG21533 Goat anti-Mouse IgM antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: C57BL/6 Mouse splenocytes stained with ARG21533 Goat anti-Mouse IgM antibody (Biotin) (pre-adsorbed) and ARG20852 anti-CD19 antibody [6D5] (PE) followed by Streptavidin (FITC).