ARG20576

Goat anti-Mouse IgM antibody (HRP), pre-adsorbed

Goat anti-Mouse IgM antibody (HRP), pre-adsorbed for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Immune System antibody

Overview

Product Description

HRP-conjugated Goat Polyclonal antibody recognizes Mouse IgM

Tested Reactivity Ms
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with the heavy chain of Mouse IgM. The antibody is pre-adsorbed with Mouse IgG and IgA, so the antibody may not react with Mouse IgG and IgA, but may react with immunoglobulins from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Mouse IgM
Species Mouse
Immunogen Mouse IgM
Target Ig IgM
Conjugation HRP
Full Name immunoglobulin heavy constant mu
Alternate Names MU; VH; AGM1; Igm; muH; Igh6; Igh-6; Igh-M;

Application Instructions

Pre Adsorbed Mouse IgG and IgA.
Application Suggestion
Tested Application Dilution
ELISA1:4000 - 1:8000
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 50% PBS (pH 7.4) and 50% Glycerol
Stabilizer 50% Glycerol
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16019 Mouse IGHM

Gene Symbol Ighm
Gene Full Name immunoglobulin heavy constant mu
Background Immunoglobulins (Ig) are the antigen recognition molecules of B cells. An Ig molecule is made up of 2 identical heavy chains and 2 identical light chains (see MIM 147200) joined by disulfide bonds so that each heavy chain is linked to a light chain and the 2 heavy chains are linked together. Each Ig heavy chain has an N-terminal variable (V) region containing the antigen-binding site and a C-terminal constant (C) region, encoded by an individual C region gene, that determines the isotype of the antibody and provides effector or signaling functions. The heavy chain V region is encoded by 1 each of 3 types of genes: V genes (see MIM 147070), joining (J) genes (see MIM 147010), and diversity (D) genes (see MIM 146910). The C region genes are clustered downstream of the V region genes within the heavy chain locus on chromosome 14. The IGHM gene encodes the C region of the mu heavy chain, which defines the IgM isotype. Naive B cells express the transmembrane forms of IgM and IgD (see IGHD; MIM 1471770) on their surface. During an antibody response, activated B cells can switch to the expression of individual downstream heavy chain C region genes by a process of somatic recombination known as isotype switching. In addition, secreted Ig forms that act as antibodies can be produced by alternative RNA processing of the heavy chain C region sequences. Although the membrane forms of all Ig isotypes are monomeric, secreted IgM forms pentamers, and occasionally hexamers, in plasma (summary by Janeway et al., 2005).[supplied by OMIM, Aug 2010]
Function IgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions (By similarity). [UniProt]
Research Area Immune System antibody

Images (1) Click the Picture to Zoom In

  • ARG20576 Goat anti-Mouse IgM antibody (HRP) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgM, IgG, and IgA. Immunoglobulins were detected with serially diluted ARG20576 Goat anti-Mouse IgM antibody (HRP) (pre-adsorbed).