ARG21550

Goat anti-Mouse Kappa Light Chain antibody (AP), pre-adsorbed

Goat anti-Mouse Kappa Light Chain antibody (AP), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Overview

Product Description AP-conjugated Goat Polyclonal antibody recognizes Mouse Kappa Light Chain
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with Mouse kappa light chains. The antibody is pre-adsorbed with Mouse lambda light chains, so the antibody may not react with Mouse lambda light chains, but may react with kappa light chains from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Kappa Light Chain
Antigen Species Mouse
Conjugation AP

Application Instructions

Pre Adsorbed Mouse lambda light chains.
Application Suggestion
Tested Application Dilution
ELISA1:2000 - 1:4000
ELISPOTAssay-dependent
FACSAssay-dependent
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 50 mM Tris (pH 8.0), 0.1% Sodium azide, 1 mM MgCl2 and 50% Glycerol
Preservative 0.1% Sodium azide
Stabilizer 50% Glycerol
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21550 Goat anti-Mouse Kappa Light Chain antibody (AP) (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG21550 Goat anti-Mouse Kappa Light Chain antibody (AP) (pre-adsorbed) followed by Red AP and hematoxylin.

  • ARG21550 Goat anti-Mouse Kappa Light Chain antibody (AP) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted ARG21550 Goat anti-Mouse Kappa Light Chain antibody (AP) (pre-adsorbed).