ARG21547

Goat anti-Mouse Kappa Light Chain antibody, pre-adsorbed

Goat anti-Mouse Kappa Light Chain antibody, pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse

Overview

Product Description Goat Polyclonal antibody recognizes Mouse Kappa Light Chain
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The antibody reacts with Mouse kappa light chains. The antibody is pre-adsorbed with Mouse lambda light chains, so the antibody may not react with Mouse lambda light chains, but may react with kappa light chains from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Kappa Light Chain
Antigen Species Mouse
Conjugation Un-conjugated

Application Instructions

Pre Adsorbed Mouse lambda light chains.
Application Suggestion
Tested Application Dilution
ELISA1:2000 - 20000
ELISPOTAssay-dependent
FACS0.1 - 1.0 μg per 10^6 cells in in a final volume of 100 μL
FLISA1:100 - 1:1000
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer BBS (pH 8.2)
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21547 Goat anti-Mouse Kappa Light Chain antibody (pre-adsorbed) IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse spleen tissue stained with ARG21547 Goat anti-Mouse Kappa Light Chain antibody (pre-adsorbed) followed by ARG21777 Donkey anti-Goat IgG (H+L) antibody (AP) (pre-adsorbed), Red AP and hematoxylin.

  • ARG21547 Goat anti-Mouse Kappa Light Chain antibody (pre-adsorbed) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21547 Goat anti-Mouse Kappa Light Chain antibody (pre-adsorbed) follow by ARG23856 Swine anti-Goat IgG (H+L) antibody (FITC) (pre-adsorbed).