Goat anti-Rabbit IgG (H+L) antibody (FITC)

Goat anti-Rabbit IgG (H+L) antibody (FITC) for Flow cytometry,ICC/IF and Rabbit

Immune System antibody
publication_link Publication2


Product Description FITC-conjugated Goat Polyclonal antibody recognizes Rabbit IgG (H+L)
Tested Reactivity Rb
Species Does Not React With Hu, Ms, Bov, Hrs
Tested Application FACS, ICC/IF
Specificity The antibody is purified on immunoaffinity rabbit IgG (H+L) column. Using ELISA, this antibody reacts with rabbit IgG (H+L), heavy and light chains and light chains of other rabbit immunoglobulins. This antibody does not cross react with non-immunoglobulin serum proteins. This antibody has minimum cross reactivity with bovine, horse, human and mouse serum proteins. Cross reactivity to other serum proteins have not been determined.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG (H+L)
Antigen Species Rabbit
Immunogen Purified Rabbit IgG (H+L)
Target Ig Rabbit IgG (H+L)
Conjugation FITC

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:1000 - 1:10000
ICC/IF1:200 - 1:1000
Application Note Note: Based on the reference DOI: 10.1016/j.matdes.2022.110581 This antibody may also suitable for IHC-P application.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Buffer 10 mM phosphate buffer (pH 7.6), 150 mM NaCl, 0.05% Sodium azide and 1% BSA.
Preservative 0.05% Sodium azide
Stabilizer 1% BSA
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Research Area Immune System antibody

Images (2) Click the Picture to Zoom In

  • ARG54745 Goat anti-Rabbit IgG (H+L) antibody (FITC) ICC/IF image

    Immunofluorescence: Neuron cells stained with ARG54745 Goat anti-Rabbit IgG (H+L) antibody (FITC) at 1:1000 dilution.

  • ARG54745 Goat anti-Rabbit IgG (H+L) antibody (FITC) ICC/IF image (Customer's Feedback)

    Immunofluorescence:Humam conjunctival stained with ARG40539 anti-NALP3 / Cryopyrin antibody at 1:200 dilution and ARG54745 Goat anti-Rabbit IgG (H+L) antibody (FITC) at 1:500 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for Goat anti-Rabbit IgG (H+L) antibody (FITC)


Sample:Humam conjunctival

Fixation Buffer:95% ethanol

Fixation Time:Overnight

Fixation Temperature:4 ºC

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:2 hr

Primary Antibody Incubation Temperature:Room Temperature ºC

Specific References

Design of macropore structure and micro-nano topography to promote the early neovascularization and osteoinductivity of biphasic calcium phosphate bioceramics

IHC-P / 

Xiangfeng Li et al.
Materials & Design,  (2022)




Mechanism behind the neuronal ephaptic coupling during synchronizing by specific brain-triggered wave as neuronal motor toolkit.

ICC/IF / Rabbit

Sajedeh Karami et al.
Sci Rep.,  (2021)